Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis

Journal of Agricultural and Food Chemistry
Tae Joung HaKeum-Yong Park

Abstract

Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans ( Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans.

References

May 16, 2002·Journal of Agricultural and Food Chemistry·Rune Slimestad, Haavard Solheim
Mar 13, 2003·Current Molecular Medicine·De-Xing Hou
Feb 19, 2004·Journal of Agricultural and Food Chemistry·Zhe ZhangJerry McLaughlin
Aug 31, 2004·The Journal of Nutrition·Séverine TalavéraChristian Rémésy
Jan 19, 2006·Journal of Agricultural and Food Chemistry·Glenda A Macz-PopJulián C Rivas-Gonzalo

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Citations

Mar 21, 2016·Plant Physiology and Biochemistry : PPB·Baolu CuiGuoping Chen
Apr 22, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guillermo Schmeda-HirschmannMaria Inés Isla
Nov 15, 2020·Electrophoresis·María Del Mar Delgado-PovedanoTim Causon
Sep 19, 2019·Journal of Agricultural and Food Chemistry·Neng-Wen YinCun-Min Qu

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