Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption Ionization- Time of Flight Mass Spectrometry

Journal of Clinical Microbiology
Ruxin LiJianhong Zhao

Abstract

Clostridium difficile multilocus sequence type 37 (ST37), which mainly corresponds to ribotype 017, has been a dominant genotype circulating in China. In this study, we report the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 204 C. difficile clinical isolates, including 49 ST37 and 155 non-ST37 isolates collected in China and other countries. The distributions of two major protein peaks (m/z 3,242 and 3,286) were significantly different between ST37 and non-ST37 prototype strains and clinical isolates. This difference was reproducible when analysis was performed on different colonies in different runs. This finding was repeated and confirmed by both bioMérieux Vitek MS and Bruker Microflex LT systems on isolates recovered from a variety of geographic regions worldwide. The combination of the two peaks was present in 47 of 49 ST37 isolates, resulting in a sensitivity of 95.9%. In contrast, the peak combination was absent in 153 of 155 non-ST37 isolates, resulting in a specificity of 98.7%. Our results suggest that MALDI-TOF MS is a rapid and reliable tool to identify C. difficile genotype ST37. Work is in progress to characterize the two molecules ...Continue Reading

References

Jun 17, 2009·Nature Reviews. Microbiology·Maja RupnikDale N Gerding
Nov 28, 2009·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·M ArvandG Bettge-Weller
Jan 1, 2010·Journal of Clinical Microbiology·David GriffithsKate E Dingle
Sep 17, 2010·Nature·Sarah A KuehneNigel P Minton
Oct 12, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Dale N Gerding
Apr 20, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M ReilS Borgmann
Jun 15, 2011·Journal of Microbiological Methods·Marie-Frédérique LartigueRoland Quentin
Jul 3, 2013·Antimicrobial Resistance and Infection Control·Deirdre A CollinsThomas V Riley
Jul 3, 2013·Journal of Microbiological Methods·Clifford G ClarkGarrett R Westmacott
Mar 14, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E ValienteB W Wren
Aug 16, 2014·International Journal of Medical Microbiology : IJMM·Marlène SaugetDidier Hocquet
Oct 30, 2016·International Journal of Antimicrobial Agents·Qiong GaoYunsong Yu
Dec 16, 2016·Journal of Clinical Microbiology·Dazhi JinYi-Wei Tang
Jan 18, 2017·Trends in Microbiology·Marlène SaugetDidier Hocquet

❮ Previous
Next ❯

Citations

May 30, 2019·Emerging Microbes & Infections·Korakrit ImwattanaThomas V Riley
Jul 18, 2019·Expert Review of Proteomics·Martin WelkerDavid Pincus
Dec 29, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thays Almeida Franco de BarcellosCarlos Henrique Camargo
May 28, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J CorverS Nicolardi
Feb 24, 2021·Anaerobe·Leandro Gouveia CarneiroEliane de Oliveira Ferreira

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.