Identification and characterization of proteolytically resistant gluten-derived peptides

Food & Function
M R Perez-GregorioV de Freitas

Abstract

The lack of digestibility of certain gluten proteins is essential in the development of celiac disease (CD). Gluten proteins are remarkably resistant to luminal and brush-border proteolysis owing to their high proline and glutamine content. Consequently, large fragments remain intact after digestion exerting toxic effects. Intestinal brush-border membrane vesicles (BBMV) have been described as having strong proteolytic activity mainly through prolyl endopeptidase enzymes. The purpose of this work was to monitor the gastrointestinal digestion of specific CD epitopes by means of an in vitro gastrointestinal digestion model that included incubation with brush-border membrane enzymes. Gluten hydrolysates were characterized by mass spectrometry and the immunologic peptides were tracked by searching the main T-cell stimulating epitopes which have been widely described. The immunologic potential of gluten hydrolysates was further analysed by enzyme-linked immunosorbent assay (ELISA). The results showed that the composition of gluten hydrolysates depended on the digestion time and protein structural characteristics. On the other hand, the main T-cell stimulating epitopes formed during hydrolysis depend on the precursor protein. Gluteni...Continue Reading

References

Jan 1, 1985·The British Journal of Nutrition·P J CiclitiraE S Lennox
Jan 1, 1986·Gene·J A Rafalski
Jan 1, 1982·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·H WieserD Idar
May 1, 1996·Acta Paediatrica. Supplement·H Wieser
Jul 4, 2001·European Journal of Gastroenterology & Hepatology·M SternH Wieser
Jan 5, 2002·Amino Acids·H J Cornell, G Wills-Johnson
Sep 28, 2002·Science·Lu ShanChaitan Khosla
Jun 5, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gianfranco MamoneFrancesco Addeo
Mar 6, 2012·Endocrine, Metabolic & Immune Disorders Drug Targets·Alessandra CamarcaCarmen Gianfrani
Mar 20, 2013·Journal of Molecular Biology·Alexei A AdzhubeiAlexander A Makarov
Jan 28, 2014·International Journal of Medical Sciences·Gorka LarrinagaJosé I López

❮ Previous
Next ❯

Citations

Jul 22, 2020·Food & Function·Amelia Torcello-GómezAlan Mackie
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Fred BrounsDaisy Jonkers
Feb 18, 2021·Food & Function·Wenjing ChenJihong Huang
Nov 3, 2021·Journal of Agricultural and Food Chemistry·Maria Rosa Pérez-GregorioVictor de Freitas

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
enzyme-linked immunosorbent assay
protein assay
ELISA
PCA

Software Mentioned

Xcalibur ( LTQ - XL )
Proteome Discoverer
STATGRAPHICS Centurion XVI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.