PMID: 8603036Jan 1, 1996Paper

Identification and characterization of transcriptional regulatory elements of the human aromatase cytochrome P450 gene (CYP19)

The Journal of Steroid Biochemistry and Molecular Biology
K TodaY Shizuta

Abstract

Aromatase cytochrome P450, a member of the cytochrome P450 gene super family, catalyzes conversion of androgens to estrogens in a form of an enzyme-complex with NADPH-cytochrome P450 reductase. Transcription of the aromatase cytochrome P450 gene (CYP19) is regulated in part by tissue-specific promoters coupled with alternative splicing mechanisms. The transcription in human placenta is governed by a promoter activity of the 5' flanking region of exon I.1, which is mapped more than 40 kb upstream from the translational start codon observed in exon II. Transient expression analyses with chimeric constructs containing the 5' flanking sequences linked to the bacterial chloramphenicol acetyltransferase (CAT) gene in human BeWo choriocarcinoma cells localized a cell-type specific enhancer element between -242 and -166 relative to the major cap site. DNase I footprinting and transient expression analyses of the enhancer element indicate that it consists of two sub-elements and that both sub-elements are necessary for the maximum enhancement of the transcription. In addition to the enhancer element, a cis-acting element important for transcriptional enhancement of the gene in response to 12-O-tetradecanoylphorbol 13-acetate in BeWo cel...Continue Reading

References

Jan 1, 1992·Molecular and Cellular Endocrinology·M W KilgoreE R Simpson
Oct 24, 1990·European Journal of Biochemistry·K TodaY Sagara
Jan 15, 1990·Biochemical and Biophysical Research Communications·N HaradaY Takagi
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Mar 1, 1982·The Biochemical Journal·M AkhtarJ N Wright
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·K TodaY Shizuta
Feb 15, 1994·Biochemical and Biophysical Research Communications·S HondaY Takagi

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Citations

Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·T YanaseH Nawata
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Nobuhiro HaradaShin-ichiro Honda
Mar 15, 2002·Trends in Endocrinology and Metabolism : TEM·Amrita KamatCarole R Mendelson
Sep 24, 2004·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Jessica RaddeAna-Maria Bamberger
Sep 26, 1997·Genes & Development·E SterneckP F Johnson
May 9, 2002·Drug Metabolism Reviews·Slobodan Rendic
Sep 4, 2007·Toxicology Letters·Yun Wang, Lai K Leung
Mar 17, 2018·Endocrinology·Sribalasubashini MuralimanoharanCarole R Mendelson
Feb 24, 2007·Journal of Medicinal Chemistry·Bin SuRobert W Brueggemeier

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