PMID: 9422603Jan 9, 1998Paper

Identification and characterization of sporulation-dependent promoters upstream of the enterotoxin gene (cpe) of Clostridium perfringens

Journal of Bacteriology
Y Zhao, Stephen B Melville

Abstract

Three promoter sites (P1, P2, and P3) responsible for the sporulation-associated synthesis of Clostridium perfringens enterotoxin, a common cause of food poisoning in humans and animals, were identified. Nested and internal deletions of the cpe promoter region were made to narrow down the location of promoter elements. To measure the effects of the deletions on the expression of cpe, translational fusions containing the promoter deletions were made with the gusA gene of Escherichia coli, which codes for beta-glucuronidase; E. coli-C. perfringens shuttle vectors carrying the fusions were introduced into C. perfringens by electroporation. In addition, in vitro transcription assays were performed with the cpe promoter region as the DNA template for extracts made from sporulating cells. DNA sequences upstream of P1 were similar to consensus SigK-dependent promoters, while P2 and P3 were similar to consensus SigE-dependent promoters. SigE and SigK are sporulation-associated sigma factors known to be active in the mother cell compartment of sporulating cells of Bacillus subtilis, the same compartment in which enterotoxin is synthesized in C. perfringens.

References

Sep 1, 1988·Molecular Microbiology·T Garnier, S T Cole
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K L Brown, H R Whiteley
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A JeffersonD Hirsh
Apr 1, 1972·Journal of Bacteriology·C L DuncanM Sebald
Mar 1, 1995·Microbiological Reviews·W G Haldenwang

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Citations

Feb 10, 2004·Applied and Environmental Microbiology·Lothar FeustelPeter Dürre
Dec 18, 2007·Journal of Bacteriology·Daniel Paredes-SabjaMahfuzur R Sarker
Apr 29, 2008·Applied and Environmental Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Apr 14, 2009·Applied and Environmental Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Nov 26, 2010·Applied and Environmental Microbiology·Andrea H HartmanStephen B Melville
Apr 16, 2013·Journal of Bacteriology·Kaori OhtaniTohru Shimizu
Jan 19, 1999·Annual Review of Microbiology·J I Rood
Apr 2, 2008·Applied and Environmental Microbiology·Benjamin OrsburnDavid L Popham
Apr 17, 2012·Microbes and Environments·Kazuaki MiyamotoBruce A McClane
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·N Mani, B Dupuy
Jul 16, 2002·Infection and Immunity·Kazuaki MiyamotoBruce A McClane
Sep 8, 2006·Applied and Environmental Microbiology·I-Hsiu Huang, Mahfuzur R Sarker
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Jihong LiBruce A McClane
Jul 30, 2011·Journal of Medical Microbiology·Daniel Paredes-Sabja, Mahfuzur R Sarker
Jun 6, 2009·Future Microbiology·Daniel Paredes-Sabja, Mahfuzur R Sarker
Jan 12, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ben WadeRobert J Moore
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Sep 1, 2008·Microbial Biotechnology·Janine R HutchisonJanine E Trempy
Apr 28, 2005·Molecular Microbiology·Didier VingadassalomIsabelle Podglajen
Jun 5, 2007·Molecular Microbiology·Susana MatamourosBruno Dupuy

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