PMID: 9440527Jan 24, 1998Paper

Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency

Journal of Bacteriology
G ZhangS R Kushner

Abstract

Using a combination of both ethyl methanesulfonate and site-directed mutagenesis, we have identified a region in DNA helicase II (UvrD) from Escherichia coli that is required for biological function but lies outside of any of the seven conserved motifs (T. C. Hodgman, Nature 333:22-23, 1988) associated with the superfamily of proteins of which it is a member. Located between amino acids 403 and 409, alterations in the amino acid sequence DDAAFER lead to both temperature-sensitive and dominant uvrD mutations. The uvrD300 (A406T) and uvrD301 (A406V) alleles produce UV sensitivity at 44 degrees C but do not affect sensitivity to methyl methanesulfonate (MMS). In contrast, the uvrD303 mutation (D403AD404A) causes increased sensitivity to both UV and MMS and is dominant to uvrD+ when present at six to eight copies per cell. Several of the alleles demonstrated a strong antimutator phenotype. In addition, conjugal recombination is reduced 10-fold in uvrD303 strains. Of all of the amino acid substitutions tested, only an alanine-to-serine change at position 406 (uvrD302) was neutral. To determine the biochemical basis for the observed phenotypes, we overexpressed and purified the UvrD303 protein from a uvrD delta294 deletion background...Continue Reading

References

Sep 15, 1977·European Journal of Biochemistry·M Abdel-MonemH Hoffmann-Berling
Sep 15, 1977·European Journal of Biochemistry·M Abdel-MonemH Hoffmann-Berling
Jan 1, 1991·Annual Review of Genetics·P Modrich
Jan 1, 1990·Annual Review of Biochemistry·S W Matson, K A Kaiser-Rogers
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G T RunyonT M Lohman
Sep 11, 1990·Nucleic Acids Research· Sibghat-UllahJ E Hearst
Jan 26, 1987·Nucleic Acids Research·C A Gilchrist, D T Denhardt
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 5, 1988·Nature·T C Hodgman
Jan 1, 1973·Journal of Bacteriology·E C Siegel
Dec 1, 1974·Journal of Bacteriology·S R Kushner
Oct 1, 1969·Journal of Bacteriology·N S Willetts, A J Clark
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·V F Maples, S R Kushner
Jan 1, 1980·Molecular & General Genetics : MGG·H M Arthur, R G Lloyd
Dec 23, 1994·Science·A Sancar
Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S W MatsonJ W George
Jul 11, 1993·Nucleic Acids Research·P MorelE Cassuto
Jan 1, 1993·Journal of Bacteriology·B K Washburn, S R Kushner
Jan 1, 1996·Annual Review of Biochemistry·T M Lohman, K P Bjornson
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·A J CLARK, A D MARGULIES
Nov 1, 1943·Genetics·S E Luria, M Delbrück

❮ Previous
Next ❯

Citations

May 7, 2014·The Journal of Biological Chemistry·Matthew J MeinersSteven W Matson
Jan 30, 2007·Journal of Bacteriology·Richard C Centore, Steven J Sandler
Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wei ChengTimothy M Lohman
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sharon CantorDavid M Livingston
May 7, 2010·The Journal of Biological Chemistry·Anuradha KumariAmanda K McCullough
Sep 30, 2010·The EMBO Journal·Eric J TomkoTimothy M Lohman
Oct 30, 2019·Chemical Reviews·Sonisilpa MohapatraTaekjip Ha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.