Identification and classification of silks using infrared spectroscopy

The Journal of Experimental Biology
Maxime Boulet-AudetChris Holland

Abstract

Lepidopteran silks number in the thousands and display a vast diversity of structures, properties and industrial potential. To map this remarkable biochemical diversity, we present an identification and screening method based on the infrared spectra of native silk feedstock and cocoons. Multivariate analysis of over 1214 infrared spectra obtained from 35 species allowed us to group silks into distinct hierarchies and a classification that agrees well with current phylogenetic data and taxonomies. This approach also provides information on the relative content of sericin, calcium oxalate, phenolic compounds, poly-alanine and poly(alanine-glycine) β-sheets. It emerged that the domesticated mulberry silkmoth Bombyx mori represents an outlier compared with other silkmoth taxa in terms of spectral properties. Interestingly, Epiphora bauhiniae was found to contain the highest amount of β-sheets reported to date for any wild silkmoth. We conclude that our approach provides a new route to determine cocoon chemical composition and in turn a novel, biological as well as material, classification of silks.

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Citations

Sep 30, 2016·Biomicrofluidics·Ksenia MaximovaSaulius Juodkazis
Aug 16, 2016·Biomacromolecules·Maxime Boulet-AudetFritz Vollrath
Jul 20, 2017·Nature Communications·Ulyana ShimanovichTuomas P J Knowles
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Sep 20, 2018·Macromolecular Bioscience·John G HardyThomas R Scheibel
Mar 7, 2019·Macromolecular Rapid Communications·Xizhou LiuTuomas P J Knowles
Apr 27, 2020·Biotechnology and Applied Biochemistry·Prithvi AsapurSantosh Mahapatra
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kelvin O MosetiYasumoto Nakazawa
Aug 5, 2017·Materials·Armandas BalčytisSaulius Juodkazis
Sep 26, 2020·Biomaterials Science·Sushant SinghSudipta Seal
Dec 5, 2020·Journal of Biomolecular Structure & Dynamics·Prithvi AsapurIndrani Banerjee
Nov 30, 2020·Acta Biomaterialia·Feng WangDavid L Kaplan
Jan 23, 2021·Soft Matter·Andrea LassenbergerNiki Baccile
Dec 23, 2020·Biotechnology and Applied Biochemistry·Prithvi C AsapurIndrani Banerjee
Sep 11, 2020·International Journal of Biological Macromolecules·Anurupa GoswamiDipali Devi
Aug 2, 2021·Environmental Science and Pollution Research International·Pauline UringStéphane C Alfaro
Jul 8, 2019·ACS Biomaterials Science & Engineering·Jiajia ZhongShengjie Ling
Jan 20, 2021·ACS Biomaterials Science & Engineering·Anastasia BrifChris Holland
Nov 10, 2021·ACS Biomaterials Science & Engineering·Jose Eduardo Ulloa RojasWendel Andrade Alves

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Methods Mentioned

BETA
infrared spectroscopy
electron microscopy
X-ray
PCA

Software Mentioned

HCA
OMNIC
XLSTAT
Cladogram
VBA

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