PMID: 8951432Nov 1, 1996Paper

Identification and cloning of site C splice variants of plasma membrane Ca-ATPase in the gerbil cochlea

Hearing Research
J J Crouch, B A Schulte

Abstract

Plasma membrane Ca-ATPase (PMCA) gene products were identified in the gerbil cochlea by reverse-transcription polymerase chain reaction (RT-PCR). Cochlear cDNA was amplified using PMCA isoform-specific primers from splice site C, the calmodulin binding domain. PCR products were cloned and sequenced. The putative housekeeping PMCA genes, 1b and 4b, as expected, were present in the gerbil cochlea and shared 98.6 and 100% amino acid homology with published rat sequences, at splice site C, respectively. PMCA2b, 3a and 3b splice variants also were detected in cochlear cDNAs and shared 95, 94.3 and 98% amino acid homology with their rat counterparts. PMCA isoforms 2 and 3 have been shown to occur in highly specialized tissues, such as muscle and brain, that require finely tuned regulation of intracellular free Ca2+ levels. The presence of several isoforms and splice variants of PMCA in the cochlea most probably reflects their differential expression among the several cell types that have been shown to contain immunoreactive PMCA. This suggests that the cochlea has developed complex mechanisms that finely tune intracellular Ca2+ levels in different highly specialized cell types.

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G M ShepherdD P Corey
Aug 1, 1988·Hearing Research·K Ikeda, T Morizono
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Sep 1, 1966·The Journal of the Acoustical Society of America·D H Moscovitch, R P Gannon
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G WalkerP G Gillespie

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Citations

Sep 11, 2004·Journal of the Association for Research in Otolaryngology : JARO·J David WoodBruce L Tempel
Aug 28, 1999·Journal of Pharmacological and Toxicological Methods·G R MonteithB D Roufogalis
Jan 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·R FicarellaE Carafoli
Nov 26, 2010·International Journal of Pediatric Otorhinolaryngology·Qingguo ChenZhiyong Li
Sep 27, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yunxia Wang LundbergKenneth L Kramer
Apr 8, 2006·Brain Research·Yunxia Wang LundbergEbenezer N Yamoah
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J J CrouchB A Schulte
Aug 11, 1998·Nature Genetics·V A StreetK Noben-Trauth
Dec 2, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ramaprasad Srinivasan, W Marston Linehan
Jan 12, 2001·Physiological Reviews·E E Strehler, D A Zacharias

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