Identification and cloning of the cDNA of a Rb-associated protein RAP140a

Science in China. Series C, Life Sciences
Q LiS Ao

Abstract

Rb exerts important physiological functions in cell-cycle control, gene expression, cell differentiation, apoptosis, development and tumorigenesis by interacting with many cellular proteins. Using human partial Rb as bait, we screened a human fetal brain cDNA library through yeast two-hybrid system and obtained six novel cDNA fragments. Among them, one cDNA fragment corresponds to two different transcripts, 7 kb and 9 kb in Northern blot analysis. These two transcripts showed uniform distribution in various human tissues. We cloned the full-length cDNA of a 7.2 kb transcript through three times PCR amplifications. It was namedRAP140a and predicted to encode a 1 233 amino acids hydrophilic protein.RAP140a was mapped to chromosome 3p13-p14. 1. RAP140a may be functionally related to the intracellular translocation of Rb or other proteins.

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Citations

May 2, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Sarah K HartenTrevor Epp
Jul 9, 2016·Clinical and Experimental Hypertension : CHE·Kimberley CrespoAlan Y Deng
May 22, 2019·Experimental Cell Research·Veronika GresakovaRadislav Sedlacek
Jul 4, 2021·Experimental Cell Research·Veronika GresakovaRadislav Sedlacek

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