Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs

RNA
Debabrata MandalU L RajBhandary

Abstract

Most archaea and bacteria use a modified C in the anticodon wobble position of isoleucine tRNA to base pair with A but not with G of the mRNA. This allows the tRNA to read the isoleucine codon AUA without also reading the methionine codon AUG. To understand why a modified C, and not U or modified U, is used to base pair with A, we mutated the C34 in the anticodon of Haloarcula marismortui isoleucine tRNA (tRNA2(Ile)) to U, expressed the mutant tRNA in Haloferax volcanii, and purified and analyzed the tRNA. Ribosome binding experiments show that although the wild-type tRNA2(Ile) binds exclusively to the isoleucine codon AUA, the mutant tRNA binds not only to AUA but also to AUU, another isoleucine codon, and to AUG, a methionine codon. The G34 to U mutant in the anticodon of another H. marismortui isoleucine tRNA species showed similar codon binding properties. Binding of the mutant tRNA to AUG could lead to misreading of the AUG codon and insertion of isoleucine in place of methionine. This result would explain why most archaea and bacteria do not normally use U or a modified U in the anticodon wobble position of isoleucine tRNA for reading the codon AUA. Biochemical and mass spectrometric analyses of the mutant tRNAs have led ...Continue Reading

References

Jan 1, 1978·Nucleic Acids Research·R E LockardU L RajBhandary
Jan 1, 1979·Methods in Enzymology·M SilberklangU L RajBhandary
Aug 10, 1979·Nucleic Acids Research·R C Gupta, K Randerath
Aug 10, 1979·Nucleic Acids Research·Y KuchinoS Nishimura
Aug 1, 1977·Nucleic Acids Research·H Donis-KellerW Gilbert
Jul 6, 1978·Nature·J Stanley, S Vassilenko
Oct 1, 1977·Nature·A SimoncsitsH Guilley
Dec 1, 1990·Journal of Bacteriology·D T Nieuwlandt, C J Daniels
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W L Lam, W F Doolittle
Feb 20, 1970·Biochemical and Biophysical Research Communications·K MuraoS Nishimura
Aug 1, 1966·Journal of Molecular Biology·F H Crick
Oct 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth WatersMichiel Noordewier
May 17, 2005·FEBS Letters·Lennart RandauDieter Söll
Aug 4, 2007·Methods in Enzymology·Takeo Suzuki, Tsutomu Suzuki
Feb 5, 2008·Methods : a Companion to Methods in Enzymology·Caroline Köhrer, Uttam L Rajbhandary
Jun 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·James G ElkinsKarl O Stetter
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Debabrata MandalUttam L RajBhandary
Feb 9, 2010·Nature Chemical Biology·Yoshiho IkeuchiTsutomu Suzuki
Apr 8, 2011·Current Opinion in Microbiology·Gabriela Phillips, Valérie de Crécy-Lagard
Jan 9, 2013·Nucleic Acids Research·Takaaki TaniguchiTsutomu Suzuki

❮ Previous
Next ❯

Citations

Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Arpita BhattacharyaUttam L RajBhandary
Nov 7, 2017·Nucleic Acids Research·Pietro BoccalettoJanusz M Bujnicki
Apr 7, 2018·Angewandte Chemie·Christina Dal MagroMark Helm
Oct 9, 2018·Chembiochem : a European Journal of Chemical Biology·Song MaoJia Sheng
Feb 13, 2019·Journal of Bacteriology·Ningxi YuPatrick A Limbach
Apr 18, 2020·Wiley Interdisciplinary Reviews. RNA·Phillip J McCownJessica A Brown
Mar 5, 2021·Nature Reviews. Molecular Cell Biology·Tsutomu Suzuki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.