Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing

Scientific Reports
Xiang-Dong ZiLi Ma

Abstract

The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present study provides the first comparison of the ovarian miRNAs of prolific Jintang black goats (JTGs) and non-prolific Tibetan goats (TBGs) during the follicular phase using RNA-Seq technology. We generated 11.19 million (M) and 11.34 M clean reads from the TBG and JTG libraries, respectively, from which a total of 389 known miRNAs were identified and 142 novel miRNAs were predicted. A total of 191 miRNAs were differentially expressed between the two breeds. Among the 10 most abundant miRNAs, miR-21-5p was defined as differentially expressed miRNA with a higher level in the JTG library than in the TBG library, but the other miRNAs were not different between the breeds. The predicted miRNA-targeted genes were further analyzed by Gene Ontology and KEGG pathway analyses. The results revealed that miR-21, miR-99a, miRNA-143, let-7f, miR-493 and miR-200b may affect follicular development. These findings will increase the current understanding of the rol...Continue Reading

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Citations

May 9, 2018·Stem Cells and Development·Elena Gonzalez-Munoz, Jose B Cibelli
Nov 25, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Dawit TesfayeKarl Schellander
May 29, 2018·Reproductive Biology and Endocrinology : RB&E·Jing YeJie Zhou
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Oct 25, 2020·Reproduction in Domestic Animals = Zuchthygiene·Pengyan SongChenyu Tao
Aug 18, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yakun WangXinping Zhu
Nov 20, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Yanjun ZhangMingjiu Luo

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Methods Mentioned

BETA
RNA-Seq
RNA Assay
PCR

Software Mentioned

KAAS webserver
miRanda
Primer3web
Solexa
cBot Cluster Generation System
R package
DEGseq
Mireap
InterProScan
PITA

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