Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Scientific Reports
Shao-Hua GuYong-Jun Zhang

Abstract

Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both i...Continue Reading

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Citations

Nov 6, 2020·Journal of Chemical Ecology·Angel Llopis-GiménezCristina M Crava
Jan 23, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Chunli XiuYongjun Zhang
Dec 24, 2018·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Tianzi GuDejun Hao
Feb 27, 2021·Bulletin of Entomological Research·Lu-Lu LiYa-Nan Zhang
Mar 12, 2021·Bulletin of Entomological Research·Xiang ZhouZhidong Li
Mar 12, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ya XingDe-Fu Chi

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Datasets Mentioned

BETA
KP331511
KP331523
KT192030
KT192054
SRR2032109
SRR2032110

Methods Mentioned

BETA
454 sequencing
PCR

Software Mentioned

MEME
SeqClean
Primer3web
CAP3
SPSS Statistics
LUCY
ClustalX
MEGA
GeneDoc
Hiseq2000

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