Identification and Comparison of Candidate Olfactory Genes in the Olfactory and Non-Olfactory Organs of Elm Pest Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) Based on Transcriptome Analysis

PloS One
Yinliang WangBingzhong Ren

Abstract

The leaf beetle Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) is a predominant forest pest that causes substantial damage to the lumber industry and city management. However, no effective and environmentally friendly chemical method has been discovered to control this pest. Until recently, the molecular basis of the olfactory system in A. quadriimpressum was completely unknown. In this study, antennae and leg transcriptomes were analyzed and compared using deep sequencing data to identify the olfactory genes in A. quadriimpressum. Moreover, the expression profiles of both male and female candidate olfactory genes were analyzed and validated by bioinformatics, motif analysis, homology analysis, semi-quantitative RT-PCR and RT-qPCR experiments in antennal and non-olfactory organs to explore the candidate olfactory genes that might play key roles in the life cycle of A. quadriimpressum. As a result, approximately 102.9 million and 97.3 million clean reads were obtained from the libraries created from the antennas and legs, respectively. Annotation led to 34344 Unigenes, which were matched to known proteins. Annotation data revealed that the number of genes in antenna with binding functions and receptor activity was greate...Continue Reading

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Citations

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Feb 11, 2020·Frontiers in Physiology·Zhang XinxinXi Jinghui

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Datasets Mentioned

BETA
SRS876374
SRS876375

Methods Mentioned

BETA
PCR
cDNA
Illumina sequencing
reverse-transcription PCR
RNA-Seq

Software Mentioned

TGICL
MEME
Graphpad Prism
SignalP
ImageJ
MEGA
MUSCLE
Hem I
Array Designer
GraphPad

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