Abstract
The limited number of gonad-specific and gonad-related genes that have been identified in fish represents a major obstacle in the study of fish gonad development and sex differentiation. In common carp Cyprinus carpio from China's Yellow River, the ovary and testis differ in volume and weight in adult fish of the same age. Comparing sperm, egg, and somatic cell transcripts in this carp may provide insight into the mechanisms of its gonad development and sex differentiation. In the present work, gene expression patterns in the carp ovary and testis were compared using suppression subtractive hybridization. Two bidirectional subtracted complementary DNA (cDNA) libraries were analyzed in parallel using testis or ovary as testers. Eighteen nonredundant clones were identified in the male library, including 15 known cDNAs. The expression patterns of selected genes in testis and ovary were analyzed using reverse transcriptase polymerase chain reaction. Tektin-1, GAPDS, FGFIBP, IGFBP-5, and an unknown gene from the Ccmg4 clone were observed to be expressed only in testis. GSDF, BMI1b, Wt1a, and an unknown gene from the Ccme2 clone were expressed at higher levels in testis than in ovary at sexual maturity. Thirty functional expressed se...Continue Reading
References
Dec 1, 1992·Endocrinology·B P Mullaney, M K Skinner
Jul 1, 1989·Molecular Endocrinology·J ClossetG Hennen
Jan 1, 1988·Molecular and Cellular Endocrinology·R B Oonk, J A Grootegoed
Jan 1, 1988·DNA·R Voutilainen, W L Miller
Jul 1, 1983·Journal of Steroid Biochemistry·E M Ritzén
Feb 1, 1995·International Journal of Andrology·Y TajimaY Nishimune
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Apr 1, 1996·International Journal of Andrology·F Urner, D Sakkas
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·K MurataK Yamagami
Dec 24, 1997·Molecular Reproduction and Development·L Del GiaccoF Cotelli
Mar 25, 1998·Biology of Reproduction·D O BunchD A O'Brien
May 1, 1998·Development, Growth & Differentiation·H SugiyamaK Yamagami
Oct 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·G DeganiK Jackson
Aug 24, 1999·FEBS Letters·N IguchiY Nishimune
May 4, 2000·Journal of Reproduction and Fertility·W BoneT G Cooper
Dec 12, 2001·Molecular and Cellular Endocrinology·H N LimJ R Hawkins
Jan 24, 2002·Biology of Reproduction·Yefei Pang, Wei Ge
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 15, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Atsuko OtaKatsumi Koga
Jul 9, 2004·Cytogenetic and Genome Research·P M WassarmanE S Litscher
Feb 3, 2005·Molecular Phylogenetics and Evolution·Ricardo MallarinoChris D Jiggins
Mar 19, 2005·Genes & Development·Makoto TachibanaYoichi Shinkai
May 3, 2005·General and Comparative Endocrinology·D MazuraisF Le Gac
Apr 15, 2006·Biotechnology Letters·Qiang XuXiuxin Deng
Jul 13, 2006·Experimental Cell Research·Peter W SetterRichard W Linck
Citations
May 11, 2016·Journal of Biotechnology·Veronica L FariniMartín Radrizzani
Nov 5, 2016·Fish & Shellfish Immunology·Vindhya MohindraJoy Krushna Jena
Feb 15, 2017·PloS One·Chuankun ZhuHuaiyu Ding
Aug 18, 2020·Journal of Molecular Histology·Xiang WangChunxu Hai
Nov 23, 2017·Fish Physiology and Biochemistry·Yongfang JiaZhongjie Chang
Apr 12, 2021·Theriogenology·Chenling GeXiaoye Wang
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Chen-Wei Hsu, Bon-Chu Chung