Identification and comparison of macrophage-induced proteins and proteins induced under various stress conditions in Brucella abortus.

Infection and Immunity
M Rafie-KolpinJ H Wyckoff

Abstract

Brucella abortus is a facultative intracellular pathogen of cattle and humans that is capable of survival inside macrophages. In order to understand how B. abortus copes with the conditions during intracellular growth in macrophages, the protein synthesis pattern of the bacteria grown inside bovine macrophages has been compared with that of bacteria grown in the cell culture medium by two-dimensional polyacrylamide gel electrophoresis. Approximately 24 new proteins that are not detected in the bacteria grown in the cell culture medium have been induced during intracellular growth in macrophages. In contrast, approximately 50 proteins that were expressed during growth in cell culture medium were completely repressed during intracellular growth. The level of expression of 19 proteins increases while that of 54 proteins decreases during intracellular growth. To understand these results, the protein synthesis pattern of B. abortus during intracellular growth was compared with those during other stress conditions. Under each stress condition studied, several new proteins were induced that were not present during regular growth conditions. Comparison of the protein synthesis pattern of B. abortus during intracellular growth with thos...Continue Reading

References

Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
May 11, 1990·Science·N A Buchmeier, F Heffron
Dec 1, 1988·Infection and Immunity·L B CorbeilA J Winter
Nov 1, 1987·The Journal of Experimental Medicine·N R Payne, M A Horwitz
Sep 1, 1985·Microbiological Reviews·J W Moulder
Apr 1, 1995·Infection and Immunity·J Lin, T A Ficht
Apr 1, 1993·Infection and Immunity·Y Abu KwaikN C Engleberg
Apr 1, 1965·The Journal of Pathology and Bacteriology·R B FITZGEORGEH SMITH
Jul 1, 1948·Journal of Bacteriology·P Gerhardt, J B Wilson

❮ Previous
Next ❯

Citations

Apr 18, 2000·Infection and Immunity·A P Teixeira-GomesM S Zygmunt
Oct 17, 2002·Annals of the New York Academy of Sciences·Cesar V MujerVito G Delvecchio
Mar 20, 2001·Infection and Immunity·S KöhlerJ P Liautard
Aug 24, 2002·Journal of Bacteriology·Michel EschenbrennerVito G DelVecchio
Jun 17, 2000·Infection and Immunity·J A KimJ E Mayfield
Jun 17, 2000·Infection and Immunity·G N ArenasL S Mayorga
Jun 27, 2006·Proteomics·Xuan Z DingDavid L Hoover
May 14, 2009·Archives of Microbiology·M Victoria DelpinoVito G Delvecchio
Nov 9, 2000·Molecular Microbiology·P LestrateJ J Letesson
Sep 24, 1999·Electrophoresis·Y A Kwaik, O S Harb
Apr 23, 2003·Acta Biologica Hungarica·A MádiL Fésüs
May 24, 2005·Infection and Immunity·Janine M LamonicaVito G DelVecchio
Apr 29, 2000·Electrophoresis·P Cash
Feb 13, 1999·Molecular Microbiology·C Guidi-RontaniM Mock
Sep 17, 2011·Science China. Life Sciences·Zhongpeng ZhaoXiliang Wang
Aug 23, 2003·Infection and Immunity·Araceli Contreras-RodriguezAhide Lopez-Merino
Feb 15, 2001·Current Opinion in Microbiology·M L BoschiroliD O'Callaghan
Aug 10, 2002·Pathologie-biologie·Sylvie Michaux-CharachonMichel Ramuz
Feb 17, 2009·Journal of Proteome Research·Julie LamontagneEustache Paramithiotis
Aug 26, 1998·Infection and Immunity·R W StichW C Brown
Jan 15, 2005·Journal of Microbiological Methods·Jill CovertGary Splitter
Jul 11, 2006·Journal of Proteome Research·Michel EschenbrennerVito G DelVecchio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Antimicrobial Chemotherapy
P David RogersLarry S McDaniel
Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association
Masayuki Umemura, Goro Matsuzaki
Proceedings of the National Academy of Sciences of the United States of America
Shunji NakanoPeter Zuber
© 2021 Meta ULC. All rights reserved