Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains

Viruses
Feng DengGuiqing Peng

Abstract

Porcine epidemic diarrhea virus (PEDV), a member of Alphacoronavirus, has caused huge economic losses for the global pork industry recently. The spike (S) protein mediates PEDV entry into host cells. Herein, we investigated the interactions between the S protein and its receptor porcine aminopeptidase N (pAPN) or co-receptor sugars. The C-terminal domain (CTD) of the S1 domain is bound to pAPN. The prototype strain demonstrated similar receptor-binding activity compared with the variant field isolate. Three loops at the tips of the β-barrel domains did not play crucial roles in the PEDV S-pAPN association, indicating that PEDV conforms to a different receptor recognition model compared with transmissible gastroenteritis virus (TGEV), porcine respiratory CoV (PRCV), and human coronavirus NL63 (HCoV-NL63). The N-terminal domain (NTD) of the PEDV S1 domain could bind sugar, a possible co-receptor for PEDV. The prototype strain exhibited weaker sugar-binding activity compared with the variant field isolate. Strategies targeting the receptor binding domain (RBD) may be helpful for developing vaccines or antiviral drugs for PEDV. Understanding the differences in receptor binding between the prototype and the variant strains may provi...Continue Reading

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Citations

May 24, 2016·Virus Research·Samaporn TeeravechyanAnan Jongkaewwattana
Apr 2, 2017·Virus Research·Wentao LiBerend-Jan Bosch
May 26, 2017·Emerging Microbes & Infections·Yingzhu ChenWenjie Tan
May 28, 2017·Virology Journal·Xing-Yi GeYun-Zhi Zhang
May 15, 2020·Pathogens·Shasha LiHaixue Zheng
Jan 8, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Honglei ZhangHui Hu
Jun 15, 2021·Frontiers in Pharmacology·Cheorl-Ho Kim
Jul 13, 2021·Veterinary Research Communications·Qiang Liu, Huai-Yu Wang

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Datasets Mentioned

BETA
ABG89335.1

Methods Mentioned

BETA
co-immunoprecipitation assay
PCR
gel filtration
ELISA
Assay
dot-blot
pull-down
fluorescence-activated cell sorting
transfection
flow cytometry

Software Mentioned

SWISS
MODEL
ClustalW

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