Identification and developmental expression of Src+ mRNAs in Xenopus laevis

Developmental Biology
J W Collett, R E Steele

Abstract

Alternative splicing of src mRNA has been demonstrated in several vertebrate species to yield a neuron-specific form of src protein termed pp60+. The function of pp60+ is unknown. The early developmental expression pattern of src+ RNA has not been previously examined. We have identified and characterized src+ transcripts corresponding to the two src genes in Xenopus laevis using a reverse transcription/polymerase chain reaction (RT/PCR) method. Both Xenopus pp60+ proteins have a 5-amino-acid insert in contrast to the 6-amino-acid insert in fish, birds, and mammals. Src+ mRNA first appears in neural plate stage Xenopus embryos, after neural induction signaling events but prior to neural differentiation. Analysis of dissected neural plate stage embryos showed that src+ mRNAs are localized to the neural plate. These findings suggest that pp60+ may play a role in elaboration of neuron structure.

References

Nov 1, 1990·The Journal of Cell Biology·W T MattenP F Maness
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GoldenJ S Brugge
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C E GeeT M Roberts
Nov 1, 1987·Molecular and Cellular Biology·J Le BeauG Walter
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P F ManessK H Pfenninger
Sep 1, 1989·Journal of Neuroscience Research·F RaulfM Schartl
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X M YangG Walter
Sep 1, 1989·Journal of Neuroscience Research·J M Pyper, J B Bolen
Mar 11, 1985·Nucleic Acids Research·R E Steele
Jan 29, 1986·Biochemical and Biophysical Research Communications·S J Parsons, C E Creutz
Sep 1, 1986·Developmental Biology·P F ManessD W Fults
Jun 1, 1983·Molecular and Cellular Biology·P C Cotton, J S Brugge
Jul 1, 1984·Developmental Biology·B T LevyP F Maness

Citations

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·M MoreauA M Duprat
May 11, 1994·Nucleic Acids Research·Stefan StammD M Helfman
Mar 11, 2006·Biochemical and Biophysical Research Communications·Françoise RothéV Kruys
Mar 7, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I L FlinkE Morkin
Aug 13, 1999·Development, Growth & Differentiation·H OnoderaK Yamasu

Related Concepts

Metazoa
DNA Sequence
Brain Chemistry
Mini-Exon
Poly(A) Tail
Xenopus laevis
SRC gene
Proto-Oncogene Protein pp60 (c-src)
SCLTB30X
SCLTB34X

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.