Identification and developmental expression profiling of putative alkaloid biosynthetic genes in Corydalis yanhusuo bulbs

Scientific Reports
Dengqun LiaoXian'en Li

Abstract

Alkaloids in bulbs of Corydalis (C.) yanhusuo are the major pharmacologically active compounds in treatment of blood vessel diseases, tumors and various pains. However, due to the absence of gene sequences in C. yanhusuo, the genes involved in alkaloid biosynthesis and their expression during bulb development remain unknown. We therefore established the first transcriptome database of C. yanhusuo via Illumina mRNA-Sequencing of a RNA composite sample collected at Bulb initiation (Day 0), early enlargement (Day 10) and maturation (Day 30). 25,013,630 clean 90 bp paired-end reads were de novo assembled into 47,081 unigenes with an average length of 489 bp, among which 30,868 unigenes (65.56%) were annotated in four protein databases. Of 526 putative unigenes involved in biosynthesis o f various alkaloids, 187 were identified as the candidate genes involved in the biosynthesis of benzylisoquinoline alkaloids (BIAs), the only alkaloid type reported in C. yanhusuo untill now. BIAs biosynthetic genes were highly upregulated in the overall pathway during bulb development. Identification of alkaloid biosynthetic genes in C. yanhusuo provide insights on pathways and molecular regulation of alkaloid biosynthesis, to initiate metabolic en...Continue Reading

References

Dec 18, 2001·Plant Physiology·A R Fernie, L Willmitzer
Nov 9, 2002·European Journal of Biochemistry·Takashi MorishigeFumihiko Sato
Oct 11, 2005·Journal of Chromatographic Science·Yizhong CaiHarold Corke
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
May 22, 2008·Chemistry & Biodiversity·Hui-Liang LiWei-Dong Zhang
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Jul 2, 2009·The Journal of Biological Chemistry·Andreas GesellToni M Kutchan
Dec 17, 2010·Plant Cell Reports·Cesar Aza-GonzálezNeftalí Ochoa-Alejo
Apr 2, 2011·The Journal of Biological Chemistry·Lesley-Ann GiddingsSarah E O'Connor
Apr 27, 2012·Plant Physiology·Thu-Thuy T Dang, Peter J Facchini
Feb 7, 2013·Plant & Cell Physiology·Jillian M Hagel, Peter J Facchini
May 21, 2013·Phytochemistry·Evangelos C TatsisBernd Schneider
Jun 14, 2013·Scientific Reports·Takashi NakatsukaMasahiro Nishihara
Aug 21, 2013·Phytochemistry·Ralph E Dewey, Jiahua Xie
Mar 29, 2014·Planta·Guillaume A W Beaudoin, Peter J Facchini
Apr 9, 2014·Current Opinion in Plant Biology·Vincenzo De LucaFang Yu
Jun 7, 2014·Journal of Experimental Botany·Fernando de la TorreFrancisco M Cánovas
Dec 2, 2014·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Kai He, Jian-Li Gao
May 15, 2015·The New Phytologist·Samuel F BrockingtonStephen A Smith
Jun 30, 2015·Plant Molecular Biology·Punchapat SojikulJarunya Narangajavana

❮ Previous
Next ❯

Citations

Apr 13, 2017·Critical Reviews in Biotechnology·Ganapathy Sivakumar
Jul 18, 2017·Frontiers in Plant Science·Ganapathy SivakumarGregory C Phillips
Sep 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Jie XinYong-Qing Zhang

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP026491

Methods Mentioned

BETA
Illumina sequencing
cDNA library
RNA-seq
PCR

Software Mentioned

SOAPdenovo
/ soap2
BLASTx
KEGG mapper
WEGO
Blast2GO
Tblastn
ESTScan
SOAP
SOAPaligner

Related Concepts

Related Feeds

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.