Identification and distribution of plasmid-type A replicator region in rhizobia

Molecular Plant-microbe Interactions : MPMI
P A BurgosN Toro

Abstract

Sinorhizobium meliloti strain GR4 harbors two cryptic plasmids, named pRmeGR4a and pRmeGR4b in addition to the symbiotic megaplasmids. The replicator region of plasmid pRmeGR4a has been recently cloned and sequenced. By DNA hybridization, homology to former replicator region was found on plasmid pRmeGR4b as well as on other plasmids harbored by S. meliloti and S. fredii strains. In these former bacteria, a PCR product of 362 bp was generated using pRmeGR4a-repC derived primers (C1 and C2). DNA sequence analysis showed that the amplified repC fragments were closely related being classified into two subgroups designated A(I) and A(II). Similarly, a PCR product of 482 bp was obtained when primers (C3 and C5) derived from pRmeGR4a repC-upstream non-coding DNA region (IR) were used. DNA sequence analysis of the corresponding amplified products showed that, as occurs, with repC the IRs were also conserved. In addition, we designed a set of primers (P2/P4), derived from the S. meliloti and S. fredii consensus sequence encompassing the IRs and repC loci, that are able to recognize homologous plasmid-type A replicator regions in Rhizobium. By using these primers, we determined that the former replicator region is widespread in S. melilo...Continue Reading

Citations

May 16, 2000·Journal of Bacteriology·M A Ramírez-RomeroM A Cevallos
Apr 25, 2008·Plasmid·Miguel A CevallosRosa María Gutiérrez-Ríos
Mar 23, 2007·Current Opinion in Microbiology·Keith E Weaver
Oct 6, 2005·Plasmid·Robert J Watson, Roselyn Heys
Jul 12, 2005·Plasmid·Javier IzquierdoMiguel A Cevallos
Oct 17, 2012·Nature Reviews. Microbiology·Uelinton M PintoStephen C Winans

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