PMID: 9163335Apr 15, 1997Paper

Identification and distribution of proteins in isolated endosomal fractions of rat liver: involvement in endocytosis, recycling and transcytosis

The Biochemical Journal
A PolC Enrich

Abstract

1. The polypeptides of the three endosomal fractions isolated from livers of oestradiol-treated rats were analysed by two-dimensional gel electrophoresis. Silver-stained gels revealed that although the three endosomal fractions shared a generally similar pattern of approx. 120 components, qualitative and quantitative differences between the three endocytic fractions could be demonstrated. 2. The 'early' endosomes [compartment of uncoupling of receptors and ligands (CURL)] comprised the most complex fraction and contained most of the polypeptides found in the 'late' endosomes [multivesicular bodies (MVBs)] and the receptor recycling compartment (RRC). When CURL was analysed by two-dimensional gel electrophoresis after partition with Triton X-114, it showed the largest number of integral membrane polypeptides. 3. Some of the major receptors (polymeric immunoglobulin receptor, transferrin receptor, low-density lipoprotein receptor, asialoglycoprotein receptor, beta1-integrin, mannose 6-phosphate receptor, epidermal growth factor receptor and AGp110) and internalized ligands (IgA, IgG, albumin, haptoglobin, transferrin and alpha2-macroglobulin) were further studied by Western blotting. 4. The distribution of the identified receptor...Continue Reading

Citations

Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C EnrichS Jäckle
Aug 7, 2004·The Journal of Physiology·Jenifer Monks, Margaret C Neville
Apr 22, 2003·Archives of Histology and Cytology·Tomoko NashidaHiromi Shimomura
May 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A PolC Enrich
Feb 28, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Shana R DaltonCarol A Casey
Oct 29, 1999·European Journal of Biochemistry·M StarkJ Johansson
Nov 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R W Van Dyke
May 20, 2000·The Journal of Biological Chemistry·S HoffenbergB J Knoll
Sep 8, 2001·The Journal of Biological Chemistry·J HeerenU Beisiegel
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·M VergésK E Mostov

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