Abstract
Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex and poorly characterized reagent. The cross-reactivity to non-tuberculous mycobacterium species (false-positive results) has been crucial to develop a more proper antigen. In the present study, we selected six M. bovis Open Reading Frames (Mb1992, Mb2031c, Mb2319, Mb2843c, Mb2845c and Mb3212c) by in-silico analysis and evaluated them in experimental and natural infection; none of these antigens had been previously assessed as diagnostic antigens for bTB. The reactivity performance was tested in animals with both positive and negative Tuberculin Skin Test (TST) results as well as in cattle infected with Mycobacterium avium subesp. paratuberculosis (MAP). The six recombinant antigens individually induced an IFN-γ response, with overall responder frequency ranging from 18.3 to 31%. Mb2845c was the most valuable antigen with the potential to discriminate TST-positive cattle from either TST-negative or MAP infected animals. Mb2845c showed si...Continue Reading
References
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·F M Ausubel, F C Cannon
Sep 1, 1993·Veterinary Microbiology·D M CollinsG W de Lisle
Apr 25, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·F A PaolicchiiM Romano
Jun 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thierry GarnierR Glyn Hewinson
Nov 29, 2005·Veterinary Microbiology·Isabel N de Kantor, Viviana Ritacco
Mar 4, 2006·Research in Veterinary Science·R de la Rua-DomenechR S Clifton-Hadley
Sep 29, 2006·Journal of Clinical Microbiology·C AagaardJ M Pollock
Jun 4, 2008·Infection and Immunity·Ben SiddersKatie Ewer
Jul 5, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Anita L Michel
Jul 3, 2009·Clinical and Vaccine Immunology : CVI·Irene SchillerBruno Oesch
Jul 2, 2010·Journal of Clinical Microbiology·Adam O WhelanMartin H Vordermeier
Jul 16, 2010·Preventive Veterinary Medicine·C AagaardA Cataldi
Mar 18, 2011·Veterinary Microbiology·Martin VordermeierR Glyn Hewinson
Mar 8, 2012·Journal of Cellular Biochemistry·Liming HeJianping Xie
Apr 4, 2012·Emerging Infectious Diseases·Jakko van IngenDick van Soolingen
Apr 24, 2012·Proteomics·Yun Sang ChoJohn T Belisle
Jan 8, 2013·The Veterinary Record·D A AbernethyS J More
May 3, 2013·PLoS Neglected Tropical Diseases·Pedro Torres-GonzalezMiriam Bobadilla-Del-Valle
Jul 23, 2013·Clinical Proteomics·Thottethodi Subrahmanya Keshava PrasadJayasuryan Narayana
Dec 24, 2014·PloS One·Maria Luisa de la CruzJulio Alvarez
Citations
Jul 26, 2019·Journal of Clinical Microbiology·Xintao GaoTing Xin
Jul 26, 2017·Scientific Reports·Aaron L OomKevan Akrami
Mar 22, 2018·Frontiers in Veterinary Science·Ting XinHong Jia
Sep 1, 2019·Pathogens·Sara CorneliPiera Mazzone
Apr 5, 2017·Journal of Translational Medicine·Sante RopertoFranco Roperto
Jan 9, 2021·International Journal of Biological Macromolecules·A Gutiérrez-OrtegaA H Alvarez
Apr 8, 2020·Journal of Molecular Microbiology and Biotechnology·Luciana VillafañeFabiana Bigi