Abstract
Until now, antiviral therapeutic agents are still urgently required for treatment or prevention of SARS-coronavirus 2 (SCoV-2) virus infection. In this study, we established a sensitive SCoV-2 Spike glycoprotein (SP), including an SP mutant D614G, pseudotyped HIV-1-based vector system and tested their ability to infect ACE2-expressing cells. Based on this system, we have demonstrated that an aqueous extract from the Natural herb Prunella vulgaris (NhPV) displayed potent inhibitory effects on SCoV-2 SP (including SPG614 mutant) pseudotyped virus (SCoV-2-SP-PVs) mediated infections. Moreover, we have compared NhPV with another compound, Suramin, for their anti-SARS-CoV-2 activities and the mode of their actions, and found that both NhPV and Suramin are able to directly interrupt SCoV-2-SP binding to its receptor ACE2 and block the viral entry step. Importantly, the inhibitory effects of NhPV and Suramin were confirmed by the wild type SARS-CoV-2 (hCoV-19/Canada/ON-VIDO-01/2020) virus infection in Vero cells. Furthermore, our results also demonstrated that the combination of NhPV/Suramin with an anti-SARS-CoV-2 neutralizing antibody mediated a more potent blocking effect against SCoV2-SP-PVs. Overall, by using SARS-CoV-2 SP-pseudo...Continue Reading
References
Mar 1, 1992·Virology·X J YaoM A Parniak
Jan 1, 1987·Antiviral Research·E De Clercq
Dec 10, 1999·Antiviral Research·H X XuJ Blay
Aug 2, 2002·Life Sciences·Shuwen LiuShuguang Wu
Aug 16, 2005·Virology·Chad M PetitK G Kousoulas
Dec 15, 2006·Journal of Virology·Corrin E McBrideCarolyn E Machamer
Mar 16, 2007·The Journal of Biological Chemistry·Zhujun AoXiaojian Yao
Sep 20, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Xiangguo QiuSteven M Jones
May 15, 2013·Antimicrobial Agents and Chemotherapy·Liyu ChenXiaojian Yao
Mar 4, 2014·Current Opinion in Virology·V Stalin RajBart L Haagmans
Dec 8, 2015·Journal of Virological Methods·Zhujun AoXiaojian Yao
Jan 19, 2016·Antiviral Research·Xu ZhangXiaojian Yao
Mar 26, 2016·Trends in Microbiology·Shuo SuGeorge F Gao
Sep 1, 2016·Antiviral Research·Szu-Cheng KuoChang-Chi Lin
Jun 2, 2017·Journal of Biosciences·Jibin SadasivanJayasri DAS Sarma
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 6, 2020·Cell Research·Manli WangGengfu Xiao
Mar 17, 2020·The Lancet Infectious Diseases·David BaudGuillaume Favre
Mar 19, 2020·The New England Journal of Medicine·Bin CaoChen Wang
Apr 3, 2020·Nature·Roman WölfelClemens Wendtner
Apr 4, 2020·Gut·Lu LinHong Shan
Apr 7, 2020·Trends in Immunology·Shibo JiangLanying Du
Apr 11, 2020·The New England Journal of Medicine·Jonathan GreinTimothy Flanigan
May 24, 2020·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Elaheh AbdollahiSeyed M Moghadas
May 28, 2020·Journal of Neurology·Zhiqiang ZhouXu Zhao
Jun 9, 2020·IScience·Javier A JaimesGary R Whittaker
Jun 24, 2020·Frontiers in Neurology·Muhammad Umer AhmedAbdul Sattar
Jul 16, 2020·Chinese Medicine·Shahid HussainXing-Xing Fan