PMID: 7541245Jun 9, 1995Paper

Identification and evaluation of the role of endogenous tyrosine kinases in azoxymethane induction of proliferative processes in the colonic mucosa of rats

Biochimica Et Biophysica Acta
N K RelanA P Majumdar

Abstract

Although tyrosine kinases (Tyr-k) are known to play a role in regulating proliferation of normal, preneoplastic and neoplastic cells, little is known about the identity of different species of Tyr-k involved in this process. Utilizing a non-denaturing polyacrylamide gel electrophoresis system, in which the separated proteins from tissue extracts are assayed directly for Tyr-k, we attempted to identify the species of Tyr-k that may be involved in azoxymethane (AOM) induction of colonic mucosal ornithine decarboxylase (ODC) activity, an enzyme whose activity is known to rise in rapidly proliferating cells. We have observed that 5 days after a single injection of the colonic carcinogen AOM (20 mg/kg body wt) to 3-4-month old rats, a significant 230% rise in colonic mucosal proliferative activity (as evidenced by 5-bromo-2'-deoxyuridine (BrdU) immunoreactivity) was also accompanied by a 550% increase in ODC activity. This was also associated with a marked rise (140-240%) in the relative activity of Tyr-k of three mucosal proteins with MI of 165, 145 and 125 kDa. Since the molecular mass of one of the Tyr-k (165 kDa) corresponded to that of EGF-receptor (EGF-R), this led us to examine the role of EGF-R Tyr-k in AOM induction of colo...Continue Reading

References

Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Mar 30, 1986·Toxicology and Applied Pharmacology·A P MajumdarM C Geokas
Jan 1, 1988·Annual Review of Biochemistry·Y Yarden, A Ullrich
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B M SeftonW C Raschke
Jun 29, 1984·Biochemical and Biophysical Research Communications·C FurihataM Takahashi
Feb 1, 1994·The Journal of Clinical Investigation·C A CartwrightB M Egbert
Sep 3, 2010·Journal of Neurochemistry·Anton I Rosenbaum, Frederick R Maxfield

❮ Previous
Next ❯

Citations

Dec 14, 1999·The Journal of Laboratory and Clinical Medicine·J L SondeenA P Majumdar
Sep 15, 2001·Mechanisms of Ageing and Development·Z Q XiaoA P Majumdar
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Manasa KannegantiEmiko Mizoguchi
Mar 4, 2011·Environmental and Molecular Mutagenesis·Page B McKinzie, Barbara L Parsons
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jyoti NautiyalAdhip P N Majumdar
Nov 27, 2004·Mechanisms of Ageing and Development·Eva M SchmelzAdhip P N Majumdar
Feb 24, 1999·Inflammatory Bowel Diseases·P L Beck, D K Podolsky
Jun 4, 2015·European Journal of Dentistry·Nahid Mohammadzadeh AkhlaghiZohreh Khalilak
Dec 22, 1999·The American Journal of Physiology·R JaszewskiA P Majumdar
Jun 5, 1998·The American Journal of Physiology·A P Majumdar, J R Goldenring

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.