Identification and expression analysis of EDR1 -like genes in tobacco (Nicotiana tabacum ) in response to Golovinomyces orontii

PeerJ
Lei WuQingyou Xia

Abstract

ENHANCED DISEASE RESISTANCE1 (EDR1) encodes a Raf-like mitogen-activated protein kinase, and it acts as a negative regulator of disease resistance and ethylene-induced senescence. Mutations in the EDR1 gene can enhance resistance to powdery mildew both in monocotyledonous and dicotyledonous plants. However, little is known about EDR1-like gene members from a genome-wide perspective in plants. In this study, the tobacco (Nicotiana tabacum)EDR1-like gene family was first systematically analyzed. We identified 19 EDR1-like genes in tobacco, and compared them to those from Arabidopsis, tomato and rice. Phylogenetic analyses divided the EDR1-like gene family into six clades, among them monocot and dicot plants were respectively divided into two sub-clades. NtEDR1-1A and NtEDR1-1B were classified into clade I in which the other members have been reported to negatively regulate plant resistance to powdery mildew. The expression patterns of tobacco EDR1-like genes were analyzed after plants were challenged by Golovinomyces orontii, and showed that several other EDR1-like genes were induced after infection, as well as NtEDR1-1A and NtEDR1-1B. Expression analysis showed that NtEDR1-13 and NtEDR1-16 had exclusively abundant expression pat...Continue Reading

References

Jul 1, 1987·Molecular Biology and Evolution·N Saitou, M Nei
Jul 26, 2000·Trends in Plant Science·P Schulze-Lefert, J Vogel
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A FryeR W Innes
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Dingzhong Tang, Roger W Innes
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T. M. DonaldI. B. Dry
Mar 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marieke van HultenJurriaan Ton
Jul 18, 2006·Nucleic Acids Research·Timothy L BaileyWilfred W Li
Aug 10, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M LindeTh Debener
Sep 20, 2006·Journal of Cellular Biochemistry·Kuo-Chen Chou, Hong-Bin Shen
Aug 19, 2007·Analytical Biochemistry·Kuo-Chen Chou, Hong-Bin Shen
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Nov 13, 2008·The Journal of Biological Chemistry·Shunichi KosugiHiroshi Yanagawa
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shunichi KosugiHiroshi Yanagawa
May 6, 2010·Annual Review of Plant Biology·Maria Cristina Suarez RodriguezJohn Mundy
Sep 3, 2010·Plant, Cell & Environment·Xiangling ShenShiping Wang
Jul 6, 2011·Molecular Plant Pathology·Katy M ChristiansenRoger W Innes
Apr 5, 2012·Molecular Plant Pathology·Ralph DeanGary D Foster
Oct 20, 2012·Molecular Biology Reports·Xingtan ZhangQingyou Xia
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
Apr 18, 2015·Nucleic Acids Research·Jianyi Yang, Yang Zhang
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 16, 2017·The Plant Journal : for Cell and Molecular Biology·Yunwei ZhangDingzhong Tang

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Datasets Mentioned

BETA
ABR45974.1
AAU89661.2
D63396.1
AAG31142.1

Methods Mentioned

BETA
PCR

Software Mentioned

Graphpad Prism
Plant
Basic Local Alignment Search Tool Protein ( BLASTP )
SMART
GeneDoc
Premier
ClustalX
GSDS
MEME
Protparam

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