Identification and expression analysis of MATE genes involved in flavonoid transport in blueberry plants

PloS One
Li ChenYadong Li

Abstract

Multidrug and toxic compound extrusion (MATE) proteins are the most recently identified family of multidrug transporters. In plants, this family is remarkably large compared to the human and bacteria counterpart, highlighting the importance of MATE proteins in this kingdom. Here 33 Unigenes annotated as MATE transporters were found in the blueberry fruit transcriptome, of which eight full-length cDNA sequences were identified and cloned. These proteins are composed of 477-517 residues, with molecular masses ~54 kDa, and theoretical isoelectric points from 5.35 to 8.41. Bioinformatics analysis predicted 10-12 putative transmembrane segments for VcMATEs, and localization to the plasma membrane without an N-terminal signal peptide. All blueberry MATE proteins shared 32.1-84.4% identity, among which VcMATE2, VcMATE3, VcMATE5, VcMATE7, VcMATE8, and VcMATE9 were more similar to the MATE-type flavonoid transporters. Phylogenetic analysis showed VcMATE2, VcMATE3, VcMATE5, VcMATE7, VcMATE8 and VcMATE9 clustered with MATE-type flavonoid transporters, indicating that they might be involved in flavonoid transport. VcMATE1 and VcMATE4 may be involved in the transport of secondary metabolites, the detoxification of xenobiotics, or the export...Continue Reading

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Citations

May 23, 2016·Applied Microbiology and Biotechnology·Huajun LvYong Wang
Sep 1, 2017·G3 : Genes - Genomes - Genetics·Ahmad H SallamBrian J Steffenson
Dec 29, 2019·BMC Plant Biology·Laura Medina-PucheRosario Blanco-Portales
Jul 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milan SkalickyHelena Hnilickova
May 21, 2019·Journal of Experimental Botany·Neha UpadhyaySourav Datta
Feb 28, 2021·Physiologia Plantarum·Satveer KaurMonika Garg

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Datasets Mentioned

BETA
AY123769.1
AAK21273
AAL27003
SRA046311

Methods Mentioned

BETA
glycosylation
PCR
RNA-Seq

Software Mentioned

MEGA
TMHMM2
HMMTOP
Clustalx
Primer Express
ProtParam
Clustalx1
SOPMA
SignalP
Primer Premier

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