Identification and Expression Analysis of miR160 and Their Target Genes in Cucumber.

Biochemical Genetics
Yaoyao LiLin Lu

Abstract

miR160 plays a crucial role in various biological processes by regulating their target gene auxin response factor (ARF) in plants. However, little is known about miR160 and ARF in cucumber fruit expansion. Here, 4 Csa-MIR160 family members and 17 CsARFs were identified through a genome-wide search. Csa-miR160 showed a closer relationship with those in melon. Phylogenetic analysis revealed that CsARFs were divided into four classes and most of CsARFs presented a closer evolutionary relationship with those from tomato. Putative cis-elements analysis predicted that Csa-MIR160 and CsARFs were involved in light, phytohormone and stress response, which proved that they might take part in light, phytohormone and stress signaling pathway by the miR160-ARF module. In addition, CsARF5, CsARF11, CsARF13 and CsARF14 were predicted as the target genes of Csa-miR160. qRT-PCR revealed that Csa-miR160 and their target gene CsARFs were differentially expressed in differential cucumber tissues and developmental stages. Csa-miR160d was only expressed in the expanded cucumber fruit. CsARF5, CsARF11 and CsARF13 exhibited the lower expression in the expanded fruit than those in the ovary, while, CsARF14 showed the reverse trend. Our results suggeste...Continue Reading

References

Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Feb 23, 1999·Methods in Molecular Biology·M R WilkinsD F Hochstrasser
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T UlmasovT J Guilfoyle
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 22, 2002·Functional & Integrative Genomics·Margarita Garcia-HernandezPeifen Zhang
Aug 22, 2003·Nature·Javier F PalatnikDetlef Weigel
May 4, 2006·Annual Review of Plant Biology·Matthew W Jones-RhoadesBonnie Bartel
Aug 4, 2007·The Plant Journal : for Cell and Molecular Biology·Po-Pu LiuJames C Carrington
Jun 3, 2008·Journal of Experimental Botany·Feng Qing FuJing Quan Yu
Sep 10, 2008·The Plant Journal : for Cell and Molecular Biology·Maaike de JongWim H Vriezen
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Oct 19, 2010·Journal of Experimental Botany·Qian-Hao Zhu, Chris A Helliwell
Apr 25, 2013·Genetics and Molecular Research : GMR·S Q Liu, L F Hu
Apr 27, 2013·Nucleic Acids Research·Stanislav BellaousovDavid H Mathews
Jul 3, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Chien Van HaLam-Son Phan Tran
Mar 4, 2014·The Plant Journal : for Cell and Molecular Biology·Geraldo Felipe Ferreira e SilvaFabio Tebaldi Silveira Nogueira
Nov 28, 2014·Nucleic Acids Research·Noe Fernandez-PozoLukas A Mueller
Dec 3, 2014·Journal of Plant Physiology·Nattaya PinwehaJarunya Narangajavana
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
May 21, 2015·Nucleic Acids Research·Shun LiuLiang-Hu Qu
Nov 21, 2015·Journal of Molecular Biology·Minoru KanehisaKanae Morishima
Jan 24, 2016·The Plant Journal : for Cell and Molecular Biology·Subha DamodharanTzahi Arazi
Jan 1, 2015·Nature Plants·Juan José RipollMartin F Yanofsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.