Identification and expression analysis of two novel members of the Mesp family in zebrafish.

The International Journal of Developmental Biology
Stephen J CuttyFiona C Wardle

Abstract

Mesp proteins play crucial roles in the formation of heart, vasculature and somites during vertebrate embryogenesis. We have used phylogenetic and genomic analysis, combined with qRT-PCR and in situ hybridization, to characterize two novel additional mesp genes in zebrafish, mesp-ab and mesp-bb, and describe their expression pattern in wild type and segmentation mutants. Both mesp-ab and mesp-bb are expressed in early mesoderm with mesp-ab expression starting during late blastula stages and mesp-bb expression initiating later, at the end of gastrulation. During somitogenesis, both mesp genes are expressed dynamically in the anterior presomitic mesoderm. mesp-ab is expressed in presumptive somites S-I and S-II, while mesp-bb is detected in S-I, S-II and S0, with expression restricted to the rostral compartment of presumptive somites. We show that the segmentation clock program regulates expression of these newly identified zebrafish mesp genes in a similar manner to their ohnologs, mesp-aa and mesp-ba. We also present evidence that zebrafish, minnow and salmon retained these additional mesp genes after the teleost whole genome duplication, while medaka, stickleback, fugu and tetraodon did not. Finally we show that although expre...Continue Reading

Citations

Mar 14, 2013·Development·Andrew C Nelson, Fiona C Wardle
Dec 18, 2015·Development, Growth & Differentiation·Taijiro Yabe, Shinji Takada
Nov 25, 2014·Cell·Jan Philipp JunkerAlexander van Oudenaarden
Mar 28, 2006·Current Topics in Developmental Biology·Andrew P Bailey, Andrea Streit
Aug 25, 2016·Developmental Biology·Ashish R DeshwarIan C Scott
Sep 7, 2013·Developmental Biology·Rachel M WargaDonald A Kane
Jan 5, 2017·Cellular and Molecular Life Sciences : CMLS·Yu Liu
Mar 1, 2015·Development·Stefanie E WindnerStephen H Devoto

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