Identification and expression analysis of alternative splice variants of the rat Hax-1 gene

Gene
Ewa A GrzybowskaJanusz A Siedlecki

Abstract

Hax-1 protein, which has been studied in mice and humans, shows a potent anti-apoptotic activity and is involved in regulation of cell motility. Cloning of the rat Hax-1 cDNA has revealed seven alternative transcripts, which differ mostly in their 5' region. Alternative splicing concerns exon 1, skipped in 5 transcripts, intron 1 which is partially retained in these transcripts, exon 2, which can be partially skipped, and intron 2, retained in one variant. The existence of different splicing variants was confirmed by exon-junction-specific RT-PCR and RNase protection assay. Analysis of expression indicates that overall Hax-1 mRNA level is relatively low in most tissues and very high in testes, and that the expression pattern of the variants is similar in different tissues. Presence of different transcripts implies the existence of several protein isoforms, with three putative start codons. The existence of at least three protein isoforms was confirmed by Western blot. Interestingly, high mRNA level in testes does not translate into high protein level, suggesting the existence of tissue-specific translational regulation or regulated protein degradation.

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Citations

Jul 26, 2012·BMC Cell Biology·Bin LiGuanghui Wang
Jul 17, 2010·BMC Research Notes·Katarzyna KokoszyńskaLucjan S Wyrwicz
Jun 16, 2009·Biochimica Et Biophysica Acta·Bengt Fadeel, Ewa Grzybowska
May 13, 2008·Journal of Molecular Biology·Delphine M LeesJohn F Marshall
Feb 9, 2011·Journal of Cellular Physiology·Solomon V YapAikaterini Kontrogianni-Konstantopoulos
Nov 21, 2012·The FEBS Journal·Ewa A GrzybowskaJanusz A Siedlecki
Sep 25, 2010·European Journal of Immunology·Doris Peckl-SchmidGernot Achatz
Jan 24, 2007·Journal of Molecular Biology·Elizabeth VafiadakiAikaterini Kontrogianni-Konstantopoulos
Jul 4, 2006·Gene·Andreas HippeMarkus Walz
Apr 14, 2015·Cellular & Molecular Immunology·Susanne WolkerstorferGertrude Achatz-Straussberger
Nov 17, 2009·Journal of Molecular and Cellular Cardiology·Solomon V YapAikaterini Kontrogianni-Konstantopoulos
Sep 23, 2016·Apoptosis : an International Journal on Programmed Cell Death·Eltyeb AbdelwahidXuelai Luo
Dec 13, 2016·Journal of Clinical Immunology·Christoph Klein
Jul 7, 2017·Journal of Clinical Immunology·Sukru CekicSara S Kilic
Dec 19, 2013·The Journal of Biological Chemistry·Jason Koontz, Aikaterini Kontrogianni-Konstantopoulos

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