Abstract
Hax-1 protein, which has been studied in mice and humans, shows a potent anti-apoptotic activity and is involved in regulation of cell motility. Cloning of the rat Hax-1 cDNA has revealed seven alternative transcripts, which differ mostly in their 5' region. Alternative splicing concerns exon 1, skipped in 5 transcripts, intron 1 which is partially retained in these transcripts, exon 2, which can be partially skipped, and intron 2, retained in one variant. The existence of different splicing variants was confirmed by exon-junction-specific RT-PCR and RNase protection assay. Analysis of expression indicates that overall Hax-1 mRNA level is relatively low in most tissues and very high in testes, and that the expression pattern of the variants is similar in different tissues. Presence of different transcripts implies the existence of several protein isoforms, with three putative start codons. The existence of at least three protein isoforms was confirmed by Western blot. Interestingly, high mRNA level in testes does not translate into high protein level, suggesting the existence of tissue-specific translational regulation or regulated protein degradation.
References
Oct 26, 1987·Nucleic Acids Research·M Kozak
Apr 1, 1994·Trends in Biochemical Sciences·A P Geballe, D R Morris
Jan 1, 1995·Annual Review of Cell and Developmental Biology·G R Hicks, N V Raikhel
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·A R GallagherR Witzgall
Nov 14, 2000·Molecular and Cellular Biology·D R Morris, A P Geballe
May 9, 2001·Nucleic Acids Research·M W Pfaffl
Jun 20, 2001·The Journal of General Virology·M DufvaL Rymo
Sep 14, 2001·Cytokine·H YinL Weissbach
Dec 26, 2001·Journal of Virology·Tyson V SharpChris Boshoff
Oct 2, 2002·Shock·Kiho ChoDavid Greenhalgh
Dec 6, 2002·Nucleic Acids Research·May Al-MaghrebiZendra E Zehner
May 6, 2003·The Journal of Biological Chemistry·Xiaoping JinDavid R Morris
Jun 6, 2003·The Journal of Investigative Dermatology·Alireza MirmohammadsadeghUlrich R Hengge
May 26, 2004·The Journal of Biological Chemistry·Daniel F OrtizIrwin M Arias
Sep 2, 2004·The Journal of Biological Chemistry·V RadhikaN Dhanasekaran
Sep 17, 2004·The Journal of Biological Chemistry·Lucia CilentiAntonis S Zervos
Citations
Aug 15, 2009·The Journal of Biological Chemistry·Siriporn JitkaewBengt Fadeel
Jul 26, 2012·BMC Cell Biology·Bin LiGuanghui Wang
Jul 17, 2010·BMC Research Notes·Katarzyna KokoszyńskaLucjan S Wyrwicz
Dec 8, 2010·Neurochemical Research·Wei ShiJian Chen
Jun 16, 2009·Biochimica Et Biophysica Acta·Bengt Fadeel, Ewa Grzybowska
May 13, 2008·Journal of Molecular Biology·Delphine M LeesJohn F Marshall
Jun 3, 2008·Journal of Internal Medicine·G CarlssonJ-I Henter
Oct 12, 2012·Acta Paediatrica·Göran CarlssonBengt Fadeel
Feb 9, 2011·Journal of Cellular Physiology·Solomon V YapAikaterini Kontrogianni-Konstantopoulos
Nov 21, 2012·The FEBS Journal·Ewa A GrzybowskaJanusz A Siedlecki
Sep 25, 2010·European Journal of Immunology·Doris Peckl-SchmidGernot Achatz
Jan 24, 2007·Journal of Molecular Biology·Elizabeth VafiadakiAikaterini Kontrogianni-Konstantopoulos
Jul 4, 2006·Gene·Andreas HippeMarkus Walz
Dec 25, 2012·Neuroscience Letters·Jing HuJiukuan Hao
Mar 12, 2011·Expert Opinion on Therapeutic Targets·Thomas Simmen
Apr 14, 2015·Cellular & Molecular Immunology·Susanne WolkerstorferGertrude Achatz-Straussberger
Nov 17, 2009·Journal of Molecular and Cellular Cardiology·Solomon V YapAikaterini Kontrogianni-Konstantopoulos
Sep 23, 2016·Apoptosis : an International Journal on Programmed Cell Death·Eltyeb AbdelwahidXuelai Luo
Dec 13, 2016·Journal of Clinical Immunology·Christoph Klein
Jul 7, 2017·Journal of Clinical Immunology·Sukru CekicSara S Kilic
Aug 10, 2017·Molecular Medicine Reports·Hao WuXiaoxia Qiu
Dec 19, 2013·The Journal of Biological Chemistry·Jason Koontz, Aikaterini Kontrogianni-Konstantopoulos