Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng

Journal of Pharmacopuncture
Doo-Young KimJun-Hyeog Jang

Abstract

Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.

References

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Jun 28, 2011·Journal of Acupuncture and Meridian Studies·Ki-Rok KwonJun-Hyeog Jang

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Citations

Mar 18, 2015·Journal of Pharmacopuncture·Kwangho LeeKirok Kwon
Mar 1, 2013·Journal of Pharmacopuncture·Han Young-JuJang Jun-Hyeog

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Methods Mentioned

BETA
PCR

Software Mentioned

Chromas

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