Identification and expression analysis of microRNAs in medaka gonads

Gene
Weiwei QiuMingyou Li

Abstract

Gonad development is a highly regulated, coordinated biological process and increasing evidences have indicated that microRNA (miRNA) may be involved in this dynamic program. Medaka (Oryzias latipes) is a good model for reproductive research as it has distinct sex determining genes, however, research in gonadal miRNAs is lacked. In this study, two small RNA libraries from the ovaries and testes were constructed and sequenced. A total of 285 conserved and 388 novel miRNAs were obtained, among which 142 mature miRNAs were significantly (> two-fold change) up or down regulated in the testis compared to the ovary. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis showed that miR-430c, miR-26a and miR-202-5p were expressed in a gonad-specific or sex-biased pattern. Fluorescence in situ hybridization (FISH) indicated that miR-202-5p was present throughout spermatogenesis and was only detected at the early stages of oogenesis, this sex biased expression pattern suggested that miR-202-5p might be a crucial candidate in male differentiation and development. Our study provides the repertoire, a comprehensive annotation of miRNAs from gonads and a reference for functional studies of miRNAs in medaka.

Citations

Sep 11, 2018·PLoS Genetics·Stéphanie GayViolette Thermes
Sep 3, 2020·Marine Biotechnology·Maria PapadakiElena Sarropoulou
Nov 23, 2020·Gene·Peng SongMingyou Li
Mar 14, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yu ZhangMingyou Li
May 8, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Shaoyu GuoMingyou Li
Jun 21, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhisheng MaWenjing Tao
Jul 11, 2021·General and Comparative Endocrinology·Sajid M AlviChun Peng

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