Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich)

Scientific Reports
Pingan GuoBo Wang

Abstract

Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in sieve elements and companion cells, which play important roles in the maintenance of morphology, photoassimilate transportation and wound protection in higher plants, but to date, no PP2 (BnPP2) genes had been identified in ramie. Here, a total of 15 full-length BnPP2 genes were identified. These BnPP2 genes exhibited different responses to abiotic stresses. Interestingly, the BnPP2 genes are more sensitive to insect pests than to other stresses. A study of the BnPP2-15 promoter revealed that pBnPP2-15 could drive specific GUS expression in the petiole, root and stamen and could also be induced by mechanical wounding and aphid infection in transgenic Arabidopsis lines. The subcellular localization of six BnPP2 proteins showed that GFP-BnPP2-1, GFP-BnPP2-6, GFP-BnPP2-7, GFP-BnPP2-9, GFP-BnPP2-11 and GFP-BnPP2-12 were predominantly located in the cytoplasm. These results provide useful information elucidating the functions of BnPP2 genes in ramie.

References

Dec 1, 1992·The Plant Cell·D E BostwickG A Thompson
Nov 1, 1994·Plant Molecular Biology·D E BostwickG A Thompson
Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Dec 30, 1998·Trends in Biochemical Sciences·E A Van der Biezen, J D Jones
Jan 8, 1999·The Plant Cell·B GoleckiG A Thompson
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·S R CutlerC R Somerville
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Jul 5, 2001·Molecular Plant-microbe Interactions : MPMI·R A OwensB Ding
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 22, 2002·Trends in Plant Science·Aart J E van BelMichael Knoblauch
Oct 9, 2002·Genome Research·Lewis Y GeerStephen H Bryant
Jan 17, 2003·Plant Physiology·Sylvie DinantGary A Thompson
Sep 17, 2003·The Plant Journal : for Cell and Molecular Biology·F VilaineS Dinant
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Feb 10, 2006·Journal of Experimental Botany·W Fred Tjallingii
Feb 14, 2006·Journal of Experimental Botany·Torsten Will, Aart J E van Bel
Feb 25, 2006·Cell·Stephen T ChisholmBrian J Staskawicz
Aug 24, 2006·Genome Biology·Victor SolovyevDenis Vorobyev
Sep 29, 2006·Current Opinion in Plant Biology·Esther LechnerPascal Genschik
Oct 12, 2007·Journal of Experimental Botany·Ayelet OmidShmuel Wolf
Nov 26, 2008·Annual Review of Plant Biology·Robert Turgeon, Shmuel Wolf
Aug 4, 2009·Biochimica Et Biophysica Acta·Nausicaä Lannoo, Els J M Van Damme
Jun 15, 2010·Comptes rendus biologies·Sylvie DinantJulia Kehr
Sep 1, 2004·Molecular Plant Pathology·Aart J E VAN Bel, Frank Gaupels
Jul 18, 2012·Plant Cell Reports·Luzia Yuriko MiyataFrancisco de Assis Alves Mourão Filho
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 26, 2013·Biochemical and Biophysical Research Communications·Jung Ro LeeSang Yeol Lee
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
Oct 3, 2017·Briefings in Bioinformatics·Kazutaka KatohKazunori D Yamada

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
environmental stresses
RNA-seq
PCR
fluorescence microscopy

Software Mentioned

Conserved Domain Architecture Retrieval Tool ( CDART )
MAFFT
TBLASTN
Coffee
ClonExpress
Clustal X
Open Finder
MEGA
R
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.