Identification and expression of MMSA-8, and its clinical significance in multiple myeloma

Oncology Reports
Rui HeWanggang Zhang

Abstract

In our previous studies, we identified 12 multiple myeloma (MM)-associated antigens by serological analysis of tumor-associated antigens with a recombinant cDNA expression library (SEREX) on MM. MM-associated antigen-8 (MMSA-8) was one of the new antigens identified. We determined the 3'- and 5'-ends of MMSA-8 using SMART-rapid amplification of cDNA ends (RACE) and then cloned its full-length cDNA in the U266 cell line. The full cDNA sequence revealed that MMSA-8 is RPS27A-related transcript variant 1 that is specifically associated with MM. We examined its prognostic significance for the first time, by investigating the correlations between MMSA-8 expression and definite clinicopathological features. We quantitatively assessed MMSA-8 expression using qRT-PCR and western blot analysis in healthy donors and MM patients. The expression levels of MMSA-8 were upregulated with statistical significance in MM patients in contrast to those in healthy donors. The expression of MMSA-8 was also upregulated in relapsed patients compared with that in the complete remission (CR) group. Contrasting MMSA-8 expression levels in different patients with definite clinicopathological features suggested an association between MMSA-8 with unfavorable...Continue Reading

References

May 1, 1996·Trends in Biochemical Sciences·I G Wool
Feb 16, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R KönigsbergJ Drach
Mar 3, 2004·Cancer Research·Rafael FonsecaHerve Avet-Loiseau
Sep 30, 2005·Cancer Immunology, Immunotherapy : CII·F L ZhouS H Liu
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Feb 10, 2009·Journal of Proteome Research·Mireya Gonzalez-BegneJohn R Yates
Apr 14, 2009·Molecular Cell·Jonathan R Warner, Kerri B McIntosh
Apr 25, 2009·Seminars in Hematology·Marie-Christine KyrtsonisArthur R Bradwell
Jul 25, 2009·Nature Reviews. Molecular Cell Biology·David KomanderSylvie Urbé
Jan 29, 2010·Cell Cycle·Nitzan Shabek, Aaron Ciechanover
Apr 5, 2011·Cancer Immunology, Immunotherapy : CII·Larry D AndersonStanley R Riddell
May 13, 2011·The Journal of Biological Chemistry·Xiao-Xin SunMu-Shui Dai
May 21, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jacalyn RosenblattDavid Avigan
May 31, 2013·British Journal of Haematology·Silvia MangiacavalliAlessandro Corso
Sep 13, 2014·Hematology/oncology Clinics of North America·Niels W C J van de Donk, Pieter Sonneveld
Sep 16, 2014·European Journal of Haematology·Pinar TarkunAbdullah Hacihanefioglu
Sep 17, 2014·American Journal of Hematology·S Vincent Rajkumar
Jan 24, 2015·Clinical Case Reports·Cristina JoãoMaria Gomes da Silva

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Methods Mentioned

BETA
PCR
density gradient centrifugation
electrophoresis
ISS
ubiquitination

Software Mentioned

SPSS
Primer Premier
SPSS17
Basic Local Alignment Search Tool ( BLAST )
BLAST
Quantity One

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