Identification and expression profiles of ERK2 and ERK5 in large yellow croaker (Larimichthys crocea) after temperature stress and immune challenge

Fish & Shellfish Immunology
Qiao-Jing JiaCui-Luan Yao

Abstract

Fish is highly affected by many environmental stresses such as temperature and invasive infection. The extracellular signal-regulated kinase (ERK) pathway, part of the mitogen-activated protein kinase (MAPK) family, is found to act as crucial mediators for cell differentiation, proliferation and cell response to various stresses. In the present study, ERK2 (LcERK2) and ERK5 (LcERK2) were cloned and characterized from large yellow croaker, Larimichthys crocea. The full length cDNA sequence of LcERK2 was of 1910 bp, including an ORF of 1110bp encoding a polypeptide of 369 amino acids. The full length cDNA sequence of LcERK5 was of 3720bp, including an ORF of 3375bp encoding a polypeptide of 1124 amino acids. Multiple alignments showed that both LcERK2 and LcERK5 contained highly conserved TEY motif and S_TKc domain in MAPK family and the unique catalytic and active structures of ERK2 and ERK5. Subcellular localization revealed that both LcERK2 and LcERK5 expressed in the cytoplasm and cell nucleus. The expression of LcERK2 and LcERK5 were detected in most tissues of large yellow croaker, with the most predominant expression of LcERK2 in brain and LcERK5 in gill, and the weakest expression of LcERK2 in liver and LcERK5 in intestin...Continue Reading

References

Mar 16, 1995·Biochimica Et Biophysica Acta·H Heider, H R Widmer
May 26, 1995·The Journal of Biological Chemistry·G ZhouJ E Dixon
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 27, 2003·The Journal of Experimental Biology·Kyra J Cowan, Kenneth B Storey
Apr 23, 2003·Experimental Cell Research·Angela M Horgan, Philip J S Stork
Sep 24, 2004·Cellular Signalling·Marie A Bogoyevitch, Naomi W Court
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Huai-Dong SongZhu Chen
Nov 19, 2004·The Journal of Biological Chemistry·Marcus Buschbeck, Axel Ullrich
Dec 27, 2005·Cellular Signalling·Xin Wang, Cathy Tournier
Jun 16, 2006·Gene Expression Patterns : GEP·S F Gabby KrensB Ewa Snaar-Jagalska
Sep 5, 2006·FEBS Letters·S F Gabby KrensB Ewa Snaar-Jagalska
Dec 26, 2006·Biochimica Et Biophysica Acta·Jean-Claude ChambardPhilippe Lenormand
Aug 8, 2007·Journal of Insect Physiology·Yoshihiro Fujiwara, David L Denlinger
Oct 12, 2007·The Journal of Biological Chemistry·Hiroko MorimotoEisuke Nishida
Feb 19, 2008·Cellular Signalling·Rebecca S ArkellSimon J Cook
May 10, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jill M KellerEvan T Keller
Jun 3, 2008·Developmental Biology·S F Gabby KrensB Ewa Snaar-Jagalska
May 10, 2011·Fish & Shellfish Immunology·Xue-Na HuangCui-Luan Yao
Jul 20, 2011·Biochimica Et Biophysica Acta·Konstantina KatsarouUrania Georgopoulou
Aug 3, 2011·The International Journal of Biochemistry & Cell Biology·Athanasios KarapetsasAlex Galanis
Oct 25, 2011·Biochimica Et Biophysica Acta·Barbara A DrewBarbara S Beckman
May 10, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Robert Roskoski
Jul 18, 2012·Cellular Signalling·Gopika N Nithianandarajah-JonesMichael J Cross
Nov 6, 2012·Gene·Shen-Hsi YangAlan J Whitmarsh
Dec 6, 2012·Journal of Proteome Research·Bettina HahnWolf D Lehmann
Mar 5, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cui-Luan Yao, George N Somero
Jul 31, 2013·Environmental Toxicology and Pharmacology·Yao-Sheng WangZong-Gui Wu
Apr 8, 2014·Virus Research·Gopinathan Pillai SreekanthThawornchai Limjindaporn

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