Identification and functional analysis of a prokaryotic-type aspartate aminotransferase: implications for plant amino acid metabolism

The Plant Journal : for Cell and Molecular Biology
Fernando de la TorreFrancisco M Cánovas

Abstract

In this paper, we report the identification of genes from pine (PpAAT), Arabidopsis (AtAAT) and rice (OsAAT) encoding a novel class of aspartate aminotransferase (AAT, EC 2.6.1.1) in plants. The enzyme is unrelated to other eukaryotic AATs from plants and animals but similar to bacterial enzymes. Phylogenetic analysis indicates that this prokaryotic-type AAT is closely related to cyanobacterial enzymes, suggesting it might have an endosymbiotic origin. Interestingly, most of the essential residues involved in the interaction with the substrate and the attachment of pyridoxal phosphate cofactor in the active site of the enzyme were conserved in the deduced polypeptide. The polypeptide is processed in planta to a mature subunit of 45 kDa that is immunologically distinct from the cytosolic, mitochondrial and chloroplastic isoforms of AAT previously characterized in plants. Functional expression of PpAAT sequences in Escherichia coli showed that the processed precursor is assembled into a catalytically active homodimeric holoenzyme that is strictly specific for aspartate. These atypical genes are predominantly expressed in green tissues of pine, Arabidopsis and rice, suggesting a key role of this AAT in nitrogen metabolism associat...Continue Reading

References

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Citations

Jun 27, 2012·Applied Biochemistry and Biotechnology·Claudius Marondedze, Ludivine A Thomas
Nov 26, 2010·Nature Chemical Biology·Hiroshi MaedaNatalia Dudareva
Aug 21, 2008·Plant & Cell Physiology·Takashi AkihiroHiroshi Ezura
Aug 12, 2009·BMC Evolutionary Biology·Shinichiro MaruyamaHisayoshi Nozaki
Jun 7, 2014·Journal of Experimental Botany·Fernando de la TorreFrancisco M Cánovas
Feb 3, 2016·Amino Acids·Korey KilpatrickFrédéric Marsolais
Dec 3, 2015·Plant & Cell Physiology·Laure GaufichonAkira Suzuki
Mar 5, 2013·The Plant Journal : for Cell and Molecular Biology·Blanca Craven-BartleConcepción Avila
Apr 30, 2016·The Plant Journal : for Cell and Molecular Biology·Jorge El-AzazFrancisco M Cánovas
Dec 25, 2008·Human Brain Mapping·Pedro A Valdes-SosaTohru Ozaki
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mei HanTao Su
Dec 19, 2017·Chemical Reviews·Stephen A KellyBrendan F Gilmore
Nov 27, 2018·Journal of Agricultural and Food Chemistry·Edward J FlahertyGale G Bozzo

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