Identification and Functional Analysis of BmNPV-Interacting Proteins From Bombyx mori (Lepidoptera) Larval Midgut Based on Subcellular Protein Levels

Frontiers in Microbiology
Shang-Zhi ZhangJia-Ping Xu

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen causing severe economic loss. However, the molecular mechanism of silkworm resistance to BmNPV and the interactions of this virus with the host during infection remain largely unclear. To explore the virus-binding proteins of silkworms, the midgut subcellular component proteins that may interact with BmNPV were analyzed in vitro based on one- and two-dimensional electrophoresis and far-western blotting combined with mass spectrometry (MS). A total of 24 proteins were determined to be specifically bound to budded viruses (BVs) in two subcellular fractions (mitochondria and microsomes). These proteins were involved in viral transportation, energy metabolism, apoptosis and viral propagation, and they responded to BmNPV infection with different expression profiles in different resistant strains. In particular, almost all the identified proteins were downregulated in the A35 strain following BmNPV infection. Interestingly, there were no virus-binding proteins identified in the cytosolic fraction of the silkworm midgut. Two candidate proteins, RACK1 and VDAC2, interacted with BVs, as determined with far-western blotting and reverse far-western blotting. We speculated that t...Continue Reading

References

Aug 28, 1998·Science·D R Green, J C Reed
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·L ZhangH Fu
Dec 10, 1999·Molecular Biology of the Cell·D H KingsleyJ Zimmerberg
Jan 9, 2001·Trends in Cell Biology·F Frischknecht, M Way
May 10, 2001·Journal of Bacteriology·A MarkaryanA M Chakrabarty
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Tsuyoshi Nishi, Michael Forgac
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 19, 2002·Journal of Neurochemistry·A Premkumar, R Simantov
Dec 7, 2002·Bioorganic & Medicinal Chemistry·Gautam Agnihotri, Hung-wen Liu
Jul 26, 2003·Science·Emily H Y ChengStanley J Korsmeyer
Sep 13, 2003·Journal of Virology·Kangayam M PonnuvelMinoru Yamakawa
Sep 10, 2004·Annual Review of Entomology·Marian R GoldsmithHiroaki Abe
Apr 9, 2005·Cell Death and Differentiation·H ZaidV Shoshan-Barmatz
Sep 17, 2005·Journal of Bioenergetics and Biomembranes·Tatiana K RostovtsevaMarco Colombini
Dec 21, 2005·The Biochemical Journal·Miklós MándiJudit Bak
Feb 14, 2006·Cellular Microbiology·Kerstin RadtkeBeate Sodeik
Mar 4, 2006·Experimental Biology and Medicine·Keli KolegraffAftab A Ansari
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Salah Abu-HamadVarda Shoshan-Barmatz
Jun 15, 2006·Nature Reviews. Molecular Cell Biology·Jean Gruenberg, F Gisou van der Goot
Jan 16, 2007·Advances in Virus Research·Jeffery Slack, Basil M Arif
Oct 4, 2007·Nature Reviews. Molecular Cell Biology·Michael Forgac
Nov 6, 2007·Current Drug Targets·L E Volkman
Dec 15, 2007·Nature Protocols·Yuliang WuXing-Zhen Chen
Oct 23, 2008·Nucleic Acids Research·Lars J JensenChristian von Mering
Dec 18, 2008·European Journal of Immunology·Vassiliki SofraSuling Li
Mar 7, 2009·Journal of Molecular and Cellular Cardiology·Andrew P Halestrap
Jun 12, 2009·Applied Biochemistry and Biotechnology·Tianran YaoMing Yan
Jul 25, 2009·Nucleic Acids Research·Yao-Qing ShenGertraud Burger
Aug 4, 2009·Virus Research·Anurag SharmaSuresh K Mittal
Mar 4, 2010·Annual Review of Biochemistry·Jason MercerAri Helenius
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel

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Methods Mentioned

BETA
electrophoresis
PCR
glycosylation

Software Mentioned

SPSS
Mascot
STRING
GPS Explorer

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