Identification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria

Applied Microbiology and Biotechnology
K KirimuraK Kino

Abstract

Thermophilic bacteria Bacillus subtilis WU-S2B and Mycobacterium phlei WU-F1 desulfurize dibenzothiophene (DBT) and alkylated DBTs through specific cleavage of the carbon-sulfur bonds over a temperature range up to 52 degrees C. In order to identify and functionally analyze the DBT-desulfurization genes, the gene cluster containing bdsA, bdsB, and bdsC was cloned from B. subtilis WU-S2B. The nucleotide and amino acid sequences of bdsABC show homologies to those of the other known DBT-desulfurization genes and enzymes; e.g. a nucleotide sequence homology of 61.0% to dszABC of the mesophilic bacterium Rhodococcus sp. IGTS8 and 57.8% to tdsABC of the thermophilic bacterium Paenibacillus sp. A11-2. Deletion and subcloning analysis of bdsABC revealed that the gene products of bdsC, bdsA and bdsB oxidized DBT to DBT sulfone (DBTO(2)), converted DBTO(2) to 2'-hydroxybiphenyl-2-sulfinate (HBPSi), and desulfurized HBPSi to 2-hydroxybiphenyl (2-HBP), respectively. Resting cells of a recombinant Escherichia coli JM109 harboring bdsABC converted DBT to 2-HBP over a temperature range of 30-52 degrees C, indicating that the gene products of bdsABC were functional in the recombinant. The activities of DBT degradation at 50 degrees C and DBT d...Continue Reading

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Citations

Feb 4, 2006·Biodegradation·Christos MeintanisAmalia D Karagouni
Sep 8, 2009·Bioscience, Biotechnology, and Biochemistry·Takashi OhshiroYoshikazu Izumi
Jan 17, 2009·Journal of Bioscience and Bioengineering·Shusuke TakahashiKohtaro Kirimura
Aug 25, 2007·Biotechnology Advances·Mehran SoleimaniArgyrios Margaritis
May 9, 2006·Current Opinion in Biotechnology·John J Kilbane
Jul 1, 2016·World Journal of Microbiology & Biotechnology·John J Kilbane, Benjamin Stark
Nov 29, 2005·Journal of Bioscience and Bioengineering·Nobuhiko NomuraHiroo Uchiyama
Oct 20, 2005·Journal of Bioscience and Bioengineering·Toshiki FuruyaKohtaro Kirimura
Oct 11, 2019·Environmental Microbiology·Guadalupe Martín-CabelloEduardo Santero
Sep 12, 2018·Applied Microbiology and Biotechnology·Shuiquan ChenYunbo Zhang

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