Identification and functional analysis of the putative anti-apoptotic gene iap4 of Spodoptera litura nucleopolyhedrovirus.
Abstract
Baculoviruses possess two types of anti-apoptotic genes, p35 and inhibitor of apoptosis (iap). In this study, we cloned a novel IAP family member, Splt-IAP4, from Spodoptera litura nucleopolyhedrovirus (SpltNPV). ScanProsite analysis of Splt-IAP4 amino acid sequence show that it contain a baculovirus IAP repeat (BIR) motif at the N-terminal portion from 18 to 61 amino acid and a Zinc finger RING motif near its C-terminus. Baculovirus IAPs could be divided into five types from IAP1 to IAP5 according to amino acid sequence homology, so the Splt-IAP4 was grouped into IAP4 because of containing a single BIR motif. Alignment of the six IAP4s in sequenced baculovirus genomes indicated that the Splt-IAP4 gene shared 74% amino acid sequence similarity with Spodoptera littoralis NPV (SpliNPV) IAP4 higher than other baculovirus IAP4 proteins, suggesting evolutionary conservation. In transient expression assay, Splt-IAP4 was not able to suppress apoptosis induced by a mutant AcMNPV deficient in p35 (vAcAnh) infection and rescue the mutant virus replication from apoptosis in Sf-9 cells. Cell viabilities analysis showed that Splt-IAP4 could not inhibit or delay the apoptosis of Sf-9 cells induced by vAcAnh infection. In summary, Splt-IAP4 h...Continue Reading
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Apoptosis
Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis