Identification and Functional Analysis of the First Aquaporin from Striped Stem Borer, Chilo suppressalis

Frontiers in Physiology
Ming-Xing LuYu-Zhou Du

Abstract

Aquaporins are integral membrane proteins some of which form high capacity water-selective channels, promoting water permeation across cell membranes. In this study, we isolated the aquaporin transcript (CsDrip1) of Chilo suppressalis, one of the important rice pests. CsDrip1 included two variants, CsDrip1_v1 and CsDrip1_v2. Although CsDrip1_v2 sequence (>409 bp) was longer than CsDrip1_v1, they possessed the same open reading frame (ORF). Protein structure and topology of CsDrip1 was analyzed using a predicted model, and the results demonstrated the conserved properties of insect water-specific aquaporins, including 6 transmembrane domains, 2 NPA motifs, ar/R constriction region (Phe69, His194, Ser203, and Arg209) and the C-terminal peptide sequence ending in "SYDF." Our data revealed that the Xenopus oocytes expressing CsDrip1 indicated CsDrip1 could transport water instead of glycerol, trehalose and urea. Further, the transcript of CsDrip1 expressed ubiquitously but differentially in different tissues or organs and developmental stages of C. suppressalis. CsDrip1 mRNA exhibited the highest level of expression within hindgut and the third instar larvae. Regardless of pupae and adults, there were significantly different expres...Continue Reading

References

Jan 1, 1988·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·O KukalJ G Duman
Nov 1, 1996·European Journal of Biochemistry·F Le CahérecJ F Hubert
Dec 22, 1999·Journal of Molecular Biology·N BlomS Brunak
Mar 25, 2000·Science·M D AdamsJ C Venter
May 26, 2001·Pflügers Archiv : European journal of physiology·M EchevarríaG Whittembury
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·J. Bernard Heymann, Andreas Engel
Oct 5, 2002·Science·Robert A HoltStephen L Hoffman
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Apr 1, 2005·American Journal of Physiology. Cell Physiology·Nancy KaufmannJeffrey L Brodsky
Jun 2, 2005·Insect Molecular Biology·M G TyshenkoV K Walker
Aug 5, 2005·Journal of Cell Science·A S Verkman
Feb 24, 2006·Proceedings of the American Thoracic Society·Peter Agre
Apr 4, 2006·Biochimica Et Biophysica Acta·Kenichi Ishibashi
Apr 5, 2007·Nature Protocols·Tania NolanStephen A Bustin
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Mar 18, 2008·The Journal of Experimental Biology·Benjamin N PhilipRichard E Lee
May 1, 2008·Annual Review of Plant Biology·Christophe MaurelVéronique Santoni
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 3, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Ewan M CampbellAlan S Bowman
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rob HorsefieldRichard Neutze
Nov 6, 2008·Insect Biochemistry and Molecular Biology·A J ShakesbyA E Douglas
Jan 20, 2009·The Journal of Experimental Biology·Jeffrey H SpringJohn A Hamlin
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Joseph D HoRobert M Stroud
Dec 15, 2010·Insect Biochemistry and Molecular Biology·Lolita G MathewJeffrey A Fabrick
Jan 21, 2011·PloS One·Lisa L DrakeImmo A Hansen
Feb 15, 2011·Journal of Insect Physiology·Shu-Xia YiRichard E Lee
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kun LiuJason L Rasgon
Apr 19, 2011·Journal of Insect Physiology·Shin G GotoDavid L Denlinger

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Datasets Mentioned

BETA
AB178640
AB469882
AF218314
EU124628
AAAB01008984
AB433197
EU127479

Methods Mentioned

BETA
dissection
PCR
X-ray

Software Mentioned

ClustalX
NetPhosK
NetPhos
Chimera
Compute pI / MW
Translate
ORF
TMHMM
BLAST
SPSS16

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