Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus.

BMC Plant Biology
Lili GuoWeilun Yin

Abstract

CBL1 is a calcium sensor that regulates drought, cold and salt signals in Arabidopsis. Overexpression of CBL1 gene in Arabidopsis and in Ammopiptanthus mongolicus showed different tolerant activities. We are interested in understanding the molecular mechanism of the upstream region of the CBL1 gene of A. mongolicus (AmCBL1). We investigated and characterized the promoter of the AmCBL1 gene, for promoters play a very important role in regulating gene expression in eukaryotes. A 1683-bp 5' flanking region was isolated from A. mongolicus. The sequence was identified as AmCBL1 promoter. Analysis of the promoter sequence indicated a 690-bp intron and some basic cis-acting elements were related to various environmental stresses and plant hormones. To identify the functional region of the AmCBL1 promoter, five plant expression vectors fused with the GUS (beta-glucuronidase) gene, driven by series deleted fragments of AmCBL1 promoter at different lengths from -1659, -1414, -1048, -296 to -167 bp relative to the transcriptional start site were constructed and transformed into Nicotiana tabacum L. cv. 89. Functional properties of each promoter segment were examined by GUS staining and fluorescence quantitative analyses using at least thr...Continue Reading

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Citations

Mar 29, 2014·Functional & Integrative Genomics·Zhongbao ZhangJianhua Wei
May 6, 2016·Frontiers in Plant Science·Murali Krishna KoramutlaRamcharan Bhattacharya
Apr 6, 2012·Plant Signaling & Behavior·Chenxing NiuJeanmarie Verchot
Jul 21, 2020·Journal of Experimental Botany·Pierre-Nicolas Boulc'hIvan Couée
Jun 3, 2017·Frontiers in Plant Science·Avinash Mishra, Bhakti Tanna
Mar 23, 2019·Applied Microbiology and Biotechnology·Anayeli Guadalupe Beltran-AguilarVirginia Aurora Herrera-Valencia

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Methods Mentioned

BETA
environmental stress
transgenic
PCR
transgenics

Software Mentioned

DNAMAN
SPSS
PLACE

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