Identification and functional characterization of the Lactococcus lactis CodY-regulated branched-chain amino acid permease BcaP (CtrA).

Journal of Bacteriology
Chris D den HengstJan Kok

Abstract

Transcriptome analyses have previously revealed that a gene encoding the putative amino acid transporter CtrA (YhdG) is one of the major targets of the pleiotropic regulator CodY in Lactococcus lactis and Bacillus subtilis. The role of ctrA in L. lactis was further investigated with respect to both transport activity as well as CodY-mediated regulation. CtrA is required for optimal growth in media containing free amino acids as the only amino acid source. Amino acid transport studies showed that ctrA encodes a secondary amino acid transport system that is specific for branched-chain amino acids (BCAAs) (isoleucine, leucine, and valine) and methionine, which is in disagreement with its previously proposed function (a cationic amino acid transporter), which was assigned based on homology. We propose to rename CtrA BcaP, for branched-chain amino acid permease. BcaP is a member of a group of conserved transport systems, as homologs are widely distributed among gram-positive bacteria. Deletion of bcaP resulted in the loss of most of the BCAA uptake activity of L. lactis, indicating that BcaP is the major BCAA carrier of this organism. Deletion of bcaP together with a second (putative) BCAA permease, encoded by brnQ, further reduced ...Continue Reading

References

May 1, 1992·Journal of Bacteriology·C SteffesB P Rosen
Sep 1, 1992·Journal of Bacteriology·E MaguinA Gruss
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Nov 1, 1987·Journal of Bacteriology·A J DriessenW N Konings
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1995·Applied and Environmental Microbiology·V JuillardJ Richard
Jan 1, 1995·Methods in Molecular Biology·H Holo, I F Nes
Oct 1, 1996·Applied and Environmental Microbiology·P G de RuyterW M de Vos
Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Nov 27, 1996·Molecular & General Genetics : MGG·K LeenhoutsJ Kok
Sep 1, 1997·Journal of Bacteriology·B R BelitskyC Von Wachenfeldt
May 2, 2001·Genes & Development·M Ratnayake-LecamwasamA L Sonenshein
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Oct 27, 2001·Science·P GlaserP Cossart
Dec 14, 2001·Journal of Bacteriology·María FernándezRichard van Kranenburg
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
May 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alex R HoffmasterClaire M Fraser
Jul 2, 2004·Molecular Microbiology·Robert P Shivers, Abraham L Sonenshein
Dec 21, 2004·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jan 5, 2005·Journal of Bacteriology·Chris D den HengstJan Kok

❮ Previous
Next ❯

Citations

Jul 23, 2011·Journal of Industrial Microbiology & Biotechnology·Ji-Cheng WangHe-Ping Zhang
Nov 6, 2007·Nature Reviews. Microbiology·Abraham L Sonenshein
Jun 10, 2008·Applied and Environmental Microbiology·Herwig BachmannJohan E T van Hylckama Vlieg
Aug 7, 2007·Journal of Bacteriology·Brice SperandioEric Guédon
Mar 15, 2014·Applied Microbiology and Biotechnology·Kadri AllerRaivo Vilu
Sep 18, 2013·Journal of Molecular Biology·Nadia GulBert Poolman
Feb 28, 2015·Journal of Industrial Microbiology & Biotechnology·Hongmiao PanHuizhong Chen
May 5, 2011·FEMS Immunology and Medical Microbiology·Ludwig StenzJacques Schrenzel
Dec 31, 2014·Infection and Immunity·Julienne C KaiserDavid E Heinrichs
Jan 23, 2019·Environmental Microbiology·Dennis WickeFabian M Commichau
Jul 26, 2019·PloS One·Mariya TarazanovaHerwig Bachmann
Aug 24, 2017·FEMS Microbiology Reviews·Jan KokOscar P Kuipers
Feb 2, 2020·Applied and Environmental Microbiology·Chenxi HuangJan Kok
Dec 19, 2016·Frontiers in Microbiology·Muhammad AfzalSulman Shafeeq
Mar 7, 2020·Nature Communications·Jhonatan A Hernandez-ValdesOscar P Kuipers
Nov 30, 2018·Frontiers in Cellular and Infection Microbiology·Michael D HoweYusuke Minato
Aug 8, 2020·Frontiers in Microbiology·Jhonatan A Hernandez-ValdesOscar P Kuipers
May 1, 2021·Applied Microbiology and Biotechnology·Nurul Amira Mohammad MohanyHarald Janovjak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.