Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512 F

Gene
Sandra Morales-ArrietaClarita Olvera-Carranza

Abstract

A Leuconostoc mesenteroides NRRL B-512 F levansucrase gene, (levS), was isolated, sequenced and cloned in Escherichia coli. The recombinant enzyme was shown to be a fructosyltransferase producing a polymer identified by (13)C-NMR as levan. Based on sequence analysis, we found that this levansucrase is a mosaic protein, bearing structural features of glucosyltransferases in the amino and carboxy terminal regions similarly to inulosucrase from Leuconostoc citreum. The phylogenetic analysis of the C-terminal region domain of levansucrases from L. mesenteroides demonstrates that they group together into a novel putative sub-family of genes and evolved long before all other glucosyltransferases, while their catalytic domain structure is species related.

References

Jan 1, 1990·Archives of Oral Biology·R R Russell
Feb 1, 1988·Journal of Bacteriology·T Shiroza, H K Kuramitsu
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 11, 1999·FEMS Microbiology Reviews·V MonchoisP Monsan
Apr 18, 2001·Protein Science : a Publication of the Protein Society·T PonsG Chinea
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Oct 1, 2003·Nature Structural Biology·Guoyu Meng, Klaus Fütterer
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jan 18, 2005·Biochimica Et Biophysica Acta·Hee Kyoung KangJohn F Robyt
Jan 1, 1980·Biochimie·A Lopez, P Monsan
Mar 1, 1993·The New Phytologist·Nicholas A Jacques

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Citations

Jul 9, 2008·Archives of Microbiology·Florian W WaldherrRudi F Vogel
Nov 17, 2009·Journal of Industrial Microbiology & Biotechnology·Dina RairakhwadaChul Ho Kim
Nov 17, 2006·Antonie van Leeuwenhoek·Clarita OlveraAgustín López-Munguía
Apr 15, 2008·Applied and Environmental Microbiology·Munir A AnwarLubbert Dijkhuizen
Jan 17, 2012·Applied and Environmental Microbiology·Clarita OlveraAgustín López-Munguía
Feb 2, 2008·BMC Biochemistry·Sandra Del MoralAgustin Lopez Munguia
Jun 6, 2014·PloS One·Maria DimopoulouMarguerite Dols-Lafargue
Feb 13, 2009·Journal of Applied Microbiology·M L Velázquez-HernándezJ J Valdez-Alarcón
Jul 15, 2015·Applied Microbiology and Biotechnology·Wenjing LiWanmeng Mu
Dec 5, 2013·Carbohydrate Polymers·Tao ZhangBo Jiang
Dec 6, 2014·Food Microbiology·Saskia Katharina MalangChristophe Lacroix
Jun 18, 2011·Gene·Carlos J Alméciga-DíazOscar F Sánchez
May 4, 2013·International Journal of Biological Macromolecules·Imen DahechKarima Srih Belghith
May 15, 2012·International Journal of Biological Macromolecules·Imen DahechHafedh Mejdoub
Sep 28, 2017·Applied Biochemistry and Biotechnology·Sudhir P SinghAshok Pandey
Sep 15, 2017·Applied Microbiology and Biotechnology·Hua Hang
Sep 30, 2020·World Journal of Microbiology & Biotechnology·Peteris ZikmanisPavels Semjonovs
Nov 10, 2020·Frontiers in Nutrition·Ibeth Peralta-GarcíaAgustín López Munguía
Aug 29, 2021·Folia Microbiologica·Waqar AhmadMunir Ahmad Anwar

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