Abstract
Autolysis of nonstarter lactic acid bacteria (NSLAB) was favorable for the development of flavor compounds during cheese manufacture. Among these bacteria, Lb. casei was regarded as the most important microbiota involved in cheese processes. In this study, a novel autolysin named AclB was identified in the genome of Lb. casei BL23 and its modular structure was predicted through bioinformatic approaches. Subsequently, its transcription profile in the exponential phase, hydrolytic activities against cell walls, enzymatic properties under different conditions, physiological function via gene inactivation and upregulation assays, as well as potential applications to NSLAB's autolysis were fully investigated. According to the results, AclB was recognized as a species-specific cell-separating enzyme, responsible for cell separation after cell division in Lb. casei BL23. The purified AclB showed considerable hydrolyzing activities towards cell walls, indicating its enzymatic nature as peptidoglycan hydrolase, or autolysin. The highest activity of AclB was determined at pH5.0 and 37°C, and the expression vector constructed based on AclB was shown to facilitate the controlled lysis of Lb. casei BL23 hosts. In summary, this study provide...Continue Reading
References
May 1, 1992·Applied Microbiology and Biotechnology·M van de GuchteG Venema
May 15, 1991·Gene·D K ChungC A Batt
Mar 20, 1998·International Journal of Food Microbiology·M C Martínez-CuestaT Requena
Apr 17, 1999·Microbiology·P MargotD Karamata
May 8, 2004·Letters in Applied Microbiology·R Cibik, M-P Chapot-Chartier
Jun 9, 2004·Applied and Environmental Microbiology·Carine HuardMarie-Pierre Chapot-Chartier
Jun 15, 2004·International Journal of Food Microbiology·Valérie GagnaireJoëlle Léonil
Dec 22, 2004·Journal of Applied Microbiology·E G Dudley, J L Steele
May 5, 2006·Journal of Bacteriology·Emmanuelle PalumboPascal Hols
Apr 10, 2007·Microbial Cell Factories·Stéphane HazebrouckPhilippe Langella
Jul 24, 2007·Applied and Environmental Microbiology·Yulia RedkoMarie-Pierre Chapot-Chartier
Sep 11, 2007·Journal of Bacteriology·Aude AntignacAlexander Tomasz
Jul 1, 2008·International Journal of Food Microbiology·Etienne DakoRonald E Simard
Jul 29, 2008·Research in Microbiology·Séverine LayecNathalie Leblond-Bourget
Oct 22, 2008·Applied and Environmental Microbiology·Mariano Prado AcostaSandra M Ruzal
Dec 24, 2010·Journal of Molecular Microbiology and Biotechnology·Christine BäuerlVicente Monedero
Feb 24, 2012·PloS One·Ingmar J J ClaesSarah Lebeer
Mar 3, 2012·PloS One·Krzysztof RegulskiMarie-Pierre Chapot-Chartier
Oct 17, 2012·Microbial Cell Factories·Thomas RolainPascal Hols
Jul 16, 2013·International Journal of Food Microbiology·Émilie Desfossés-FoucaultDenis Roy
Jul 16, 2013·Bioengineered·Sahar NavidghasemizadPer Ej Saris
Aug 29, 2013·International Journal of Medical Sciences·Min FangXue-Yuan Liu
May 27, 2014·Microbiology·Julia AnzengruberChristina Schäffer
Aug 12, 2014·PloS One·Xiao-Yang PangJia-Ping Lv
Citations
Mar 7, 2018·Journal of Molecular Microbiology and Biotechnology·Carlos Eduardo Serrano-MaldonadoMaricarmen Quirasco
Apr 3, 2019·International Journal of Molecular Sciences·Susan J Pepper, Margaret L Britz
Jan 5, 2020·Applied Microbiology and Biotechnology·Israel García-CanoRafael Jiménez-Flores
Feb 29, 2020·Journal of Proteome Research·Kayode T AduMargaret L Britz
Oct 17, 2021·World Journal of Microbiology & Biotechnology·Adriana López-ArvizuIsrael García-Cano