Identification and functional characterization of TRPA1 in human myoblasts

Pflügers Archiv : European journal of physiology
Markus OsterlohHanns Hatt

Abstract

The proper function of the skeletal muscle is essential for the survival of most animals. Thus, efficient and rapid repair of muscular damage following injury is crucial. In recent years, satellite cells have emerged as key players of muscle repair, capable of undergoing extensive proliferation after injury, fusing into myotubes and restoring muscle function. Furthermore, it has been shown that Ca(2+)/calmodulin-dependent generation of nitric oxide (NO) is an important regulator of muscle repair. Here, we demonstrate the functional expression of transient receptor potential, subfamily A1 (TRPA1) channel in human primary myoblasts. Stimulation of these cells with well-known TRPA1 ligands led to robust intracellular Ca(2+) rises which could be inhibited by specific TRPA1 antagonists. Moreover, we show that TRPA1 activation enhances important aspects of skeletal muscle repair such as cell migration and myoblast fusion in vitro. Interestingly, TRPA1 levels and inducible Ca(2+) transients decline with ongoing myoblast differentiation. We suggest that TRPA1 might serve as a physiological mediator for inflammatory signals and appears to have a functional role in promoting myoblast migration, fusion, and potentially also in activating ...Continue Reading

References

Mar 1, 1981·Developmental Biology·J D DavidC A Higginbotham
Nov 1, 1995·Journal of Cellular Physiology·R E AllenG M Rice
Feb 1, 1994·Annals of Internal Medicine·C J LowensteinS H Snyder
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J D Molkentin, E N Olson
Apr 29, 1998·The Journal of Clinical Investigation·I RubinsteinO S Better
Jan 19, 1999·Annual Review of Cell and Developmental Biology·B L Black, E N Olson
Apr 20, 2001·The Journal of Cell Biology·V HorsleyG K Pavlath
May 22, 2003·Cell·Valerie HorsleyGrace K Pavlath
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
Jan 28, 2004·Cells, Tissues, Organs·Valerie Horsley, Grace K Pavlath
Mar 30, 2004·Neuron·Michael BandellArdem Patapoutian
Dec 25, 2004·Cellular and Molecular Life Sciences : CMLS·P BastianF Entschladen
Feb 10, 2006·Experimental Cell Research·J F LafreniereJ P Tremblay
May 11, 2006·American Journal of Physiology. Cell Physiology·Ryuichi TatsumiRonald E Allen
May 12, 2006·Journal of Cellular Physiology·Serge ArnaudeauLaurent Bernheim
Jul 26, 2006·The Journal of Cell Biology·Katie M Jansen, Grace K Pavlath
Sep 15, 2006·The International Journal of Biochemistry & Cell Biology·Ludovic LeloupJean-Jacques Brustis
Aug 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Colleen R McNamaraChristopher M Fanger
Jan 30, 2008·The Journal of Biological Chemistry·Gyu-Un BaeRobert S Krauss
Mar 13, 2008·British Journal of Pharmacology·S P LeeR W Bryant
Apr 10, 2008·The Journal of Clinical Investigation·Bret F BessacSven-Eric Jordt
Apr 12, 2008·The International Journal of Biochemistry & Cell Biology·Michiko YamadaRonald E Allen
Nov 13, 2008·Journal of Cell Science·Magali LouisPhilippe Gailly
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuji KarashimaThomas Voets
Jan 23, 2009·American Journal of Physiology. Cell Physiology·Ryuichi TatsumiRonald E Allen
Jul 4, 2009·BMC Molecular Biology·Jens Stern-StraeterUlrich R Goessler
Aug 18, 2009·Nitric Oxide : Biology and Chemistry·Lidiane Isabel FilippinRicardo Machado Xavier
Nov 20, 2009·Developmental Cell·Christine A GriffinGrace K Pavlath
Dec 17, 2009·Medicine and Science in Sports and Exercise·Gøran PaulsenTruls Raastad
Aug 26, 2010·Journal of Cell Science·Christine A GriffinGrace K Pavlath
Sep 3, 2010·PloS One·Daniel Vilceanu, Cheryl L Stucky
Nov 17, 2010·The Journal of Physiology·Bernd NiliusGrzegorz Owsianik
Nov 26, 2010·Nitric Oxide : Biology and Chemistry·Lidiane Isabel FilippinRicardo Machado Xavier
May 12, 2011·The Journal of Physiology·Stuart M BrierleyL Ashley Blackshaw
Jul 30, 2011·British Journal of Pharmacology·Laura VayPeter A McNaughton

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