Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1 ): A Potential Candidate for Reducing Pre- and Post-harvest Loss of Sucrose in Sugarcane

Frontiers in Plant Science
Suresha G ShivalingamurthyAnne L Rae

Abstract

In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play a key role in post-translation regulation of plant invertases through which sucrose hydrolysis is controlled. INVINH proteins are small (18 kDa) members of the pectin methylesterase inhibitor superfamily and they are moderately conserved across plants. In the present study, we identified two INVINH genes from sugarcane, ShINH1 and ShINH2. In silico characterization of the encoded proteins revealed 43% sequence identity at the amino acid level, confirming the non-allelic nature of the proteins. The presence of putative signal peptide and subcellular targeting sequences revealed that ShINH1 and ShINH2 likely have apoplasmic and vacuolar localization, respectively. Experimental visualization of ShINH1-GFP revealed that ShINHI is indeed exported to the apoplast. Differential tissue-specific and developmental expression of ShINH1 between leaf, stalk, flower and root suggest that it plays a role in controlling source-sink metabolic regulation during sucrose a...Continue Reading

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Datasets Mentioned

BETA
CO373080
MG457817

Methods Mentioned

BETA
transgenic
PCR
gel filtration
invertase assay
confocal microscopy
Circular dichroism

Software Mentioned

SignalP
BLASTn
PrediSi
CLC Genomics Workbench
GraphPad Prism
Mega
MEGA7
BLAST
Statistical Analysis system ( SAS )
PSIPRED

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