Identification and functional characterization of SlDronc in Spodoptera littoralis

PeerJ
Hao LiuZhouning Yang

Abstract

Apoptosis is responsible for eliminating damaged and virus-infected cells, regulating normal cell turnover, and maintaining the immune system's development and function. Caspases play a vital role in both mammal and invertebrate apoptosis. Spodoptera littoralis is a generalist insect herbivore that is one of the most destructive pests in tropical and subtropical areas and attacks a wide range of commercially important crops. Although S. littoralis is a model organism in the study of baculovirus infection, its apoptotic pathway has not been explored. We cloned a new caspase gene named sldronc in S. littoralis using Rapid Amplification of cDNA Ends (RACE). We then measured caspase activity on synthetic caspase substrates and S. littoralis' effector caspase. SlDronc's function in the apoptotic pathway and its interaction with caspase inhibitors were also tested in SL2 cells. We found that the initiator caspase SlDronc cleaved and activated effector caspase in S. littoralis. SlDronc overexpression induced apoptosis in SL2 cells, and Sldronc knockdown decreased apoptosis induced by UV irradiation in SL2 cells. Our results indicate that SlDronc acts as an apoptotic initiator caspase in S. littoralis. Additionally, we found that proce...Continue Reading

References

Apr 11, 1997·The Journal of Biological Chemistry·R V TalanianW W Wong
Jul 17, 1997·Nature·Q L DeverauxJ C Reed
Aug 15, 1997·The Biochemical Journal·G M Cohen
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L DorstynS Kumar
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G S Salvesen, V M Dixit
Sep 14, 2000·The Journal of Biological Chemistry·L M QuinnH Richardson
Jan 4, 2001·Cell Death and Differentiation·S Kumar, J Doumanis
Apr 21, 2001·Cell Death and Differentiation·R J Clem
Mar 7, 2003·Molecular Cell·Mrudula DonepudiMarkus G Grütter
Apr 15, 2003·Nature Cell Biology·Mark DitzelPascal Meier
Nov 25, 2003·Oncogene·Alexei DegterevJunying Yuan
Apr 27, 2004·Nature Structural & Molecular Biology·Nieng YanYigong Shi
Sep 24, 2004·Journal of Cell Science·Israel MuroRollie J Clem
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Stefan J Riedl, Yigong Shi
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·David L Vaux, John Silke
Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·Q LiuN Chejanovsky
Apr 25, 2006·Molecular Cell·Cristina PopGuy S Salvesen
Jul 18, 2006·Annual Review of Cell and Developmental Biology·Bruce A Hay, Ming Guo
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Dec 18, 2007·Cell Death and Differentiation·L Dorstyn, S Kumar
Jul 8, 2008·Methods in Enzymology·Cristina PopFiona L Scott
Oct 22, 2008·Oncogene·J Li, J Yuan
Nov 18, 2008·Nature Cell Biology·Peter J ShapiroHyung Don Ryoo
Nov 26, 2008·Molecular Cell·Mark DitzelPascal Meier
Feb 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Dawn M CooperCarl Lowenberger
May 29, 2009·The Journal of Biological Chemistry·Cristina Pop, Guy S Salvesen
Sep 8, 2009·Microbes and Infection·Linde DuprezPeter Vandenabeele
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Stephen W G Tait, Douglas R Green
Sep 14, 2011·Insect Biochemistry and Molecular Biology·Ikue SuganumaMotoko Ikeda
Nov 19, 2011·Cell Death and Differentiation·M Darding, P Meier
Apr 25, 2012·Annual Review of Biochemistry·Takafumi TasakiYong Tae Kwon
Mar 12, 2013·Insect Biochemistry and Molecular Biology·Ning HuangRollie J Clem
Jun 8, 2013·Biochemical and Biophysical Research Communications·Koji KitaguchiMotoko Ikeda
May 31, 2014·Trends in Cell Biology·Daniel J GibbsMichael J Holdsworth
Nov 16, 2014·Apoptosis : an International Journal on Programmed Cell Death·Yu-Chih LoJung-Yu Tung
Feb 18, 2016·Immunity·Lorenzo GalluzziGuido Kroemer

❮ Previous
Next ❯

Datasets Mentioned

BETA
ABC94941.1
AEK71902.1

Methods Mentioned

BETA
Rapid Amplification of
PCR
transfection

Software Mentioned

PsiPred
SlDronc
GraphPad Prism
InterProScan
Quantity One
MEGA

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis