PMID: 7547709May 1, 1995Paper

Identification and functional characterization of mouse CD29 with a mAb

International Immunology
K NotoH Yagita

Abstract

The beta 1 integrin subfamily, alternatively called very late activation antigen (VLA), has been implicated in various cellular functions. In this study, we generated a mAb against the mouse beta 1 subunit (CD29) to examine the functional property of mouse VLA proteins. After immunization with affinity-purified mouse VLA-4 (alpha 4 beta 1), a hamster mAb, HM beta 1-1, was established by screening mAb that reacted with alpha 4-negative neuroblastoma C1300. The antigen defined by HM beta 1-1 was widely distributed in various mouse cell lines and HM beta 1-1 immunoprecipitated a 110-120 kDa protein common to VLA-1 and VLA-6, indicating that HM beta 1-1 recognizes the beta 1 subunit of mouse integrins. We then examined the inhibitory effect of HM beta 1-1 on VLA-dependent cell adhesion and activation. HM beta 1-1 blocked the adhesion of mouse tumor cell lines to extracellular matrix proteins including collagen, laminin and fibronectin. Moreover, splenic T cell proliferation induced by anti-CD3 mAb and allogeneic mixed lymphocyte response were strongly inhibited by HM beta 1-1 in combination with an anti-LFA-1 mAb. We conclude that HM beta 1-1 reactive with mouse CD29 can inhibit VLA-dependent cellular functions and, thus, would be ...Continue Reading

Citations

Jun 9, 2005·Experimental and Molecular Pathology·Renald A Blundell, David J Harrison
Sep 15, 2001·Mechanisms of Ageing and Development·M L BurgessH L Hedrick
Sep 1, 1997·General Pharmacology·T TominagaH Shimada
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mikhail MartchenkoStanley N Cohen
Jun 15, 2011·Molecular and Cellular Biology·Jie LiuShaohua Li
Jan 21, 2006·The Journal of Clinical Investigation·Akiyoshi UemuraShin-ichi Nishikawa
May 23, 2013·Cell Communication and Signaling : CCS·Matthew P KeaseyTheo Hagg
Jul 18, 2009·Stem Cells·Ashley L SiegelD D W Cornelison
Mar 22, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolyn Saylor HawkArturo Casadevall
Apr 25, 2008·Current Protocols in Immunology·M J Puklavec, A N Barclay
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan T PribilaYoji Shimizu
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·V C RidgerP G Hellewell
May 25, 2006·Journal of Cell Science·Kyle J McQuadeAlan C Rapraeger

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