PMID: 9420252Jan 7, 1998Paper

Identification and functional characterization of a high-affinity Bel-1 DNA binding site located in the human foamy virus internal promoter

Journal of Virology
Y KangB R Cullen

Abstract

The transcription of genes carried by primate foamy viruses is dependent on two distinct promoter elements. These are the long terminal repeat (LTR) promoter, which regulates expression of the viral structural proteins, and a second internal promoter, located towards the 3' end of the env gene, that directs expression of the viral auxiliary proteins. One of these auxiliary proteins is a potent transcriptional transactivator, termed Bel-1 in human foamy virus (HFV) and Tas or Taf in the related simian foamy viruses, that is critical for foamy virus replication. Previously, it has been demonstrated that the LTR promoter element of HFV contains a DNA binding site for Bel-1 that is critical for transcriptional activation (F. He, W. S. Blair, J. Fukushima, and B. R. Cullen, J. Virol. 70:3902-3908, 1996). Here, we extended this earlier work by using methylation interference analysis to identify and characterize the Bel-1 DNA binding sites located in the HFV LTR and internal promoter elements. Based on these data, we propose a minimal, 25-bp DNA binding site for Bel-1, derived from the HFV internal promoter element, and show that this short DNA sequence mediates efficient Bel-1 binding both in vitro and in vivo. We further demonstrate...Continue Reading

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Citations

Nov 26, 2010·The Journal of General Virology·Andrea SchulzeJochen Bodem
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Sep 28, 2013·Viruses·Axel Rethwilm, Jochen Bodem
Jun 10, 2016·International Journal of Molecular Medicine·Jihui DuanGuojun Zhang
Mar 22, 2002·Journal of Virology·Martin HeinkeleinAxel Rethwilm
May 7, 2010·Medical Microbiology and Immunology·Axel Rethwilm
Dec 16, 1998·Human Gene Therapy·G D Trobridge, D W Russell
Nov 26, 2002·The Journal of Biological Chemistry·Kenji KidoRolf M Flügel
Dec 17, 2010·Journal of Virology·Jochen BodemAxel Rethwilm
Sep 28, 2012·Journal of Virology·Réjane RuaAntoine Gessain
Jun 20, 2001·Journal of Virology·C D MeieringM L Linial
Sep 28, 2004·The Journal of General Virology·Shinya OmotoYoichi R Fujii
Jul 18, 2020·PLoS Pathogens·Melissa KanePaul D Bieniasz
Feb 11, 1999·Journal of Virology·M L Linial
Jan 18, 2006·Retrovirology·Anne Saumet, Charles-Henri Lecellier

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