Identification and gene expression analyses of ghrelin in the stomach of Pacific bluefin tuna (Thunnus orientalis)

General and Comparative Endocrinology
Atsushi SudaHironori Ando

Abstract

Full length cDNA and gene encoding ghrelin precursor and mature ghrelin peptide were identified from the stomach of Pacific bluefin tuna, Thunnus orientalis, which has unique metabolic physiology and high commercial value at fishery markets. Quantitative expression analysis was conducted for the gastric ghrelin and pepsinogen 2 genes during the early stage of somatic growth from the underyearling to yearling fish. The full length cDNA of bluefin tuna ghrelin precursor has a length of 470bp and the deduced precursor is composed of 107 amino acids. The ghrelin gene is 1.9kbp in length and has a 4 exon-3 intron structure. The major form of mature ghrelin in the stomach was an octanoylated 20-amino acid peptide with C-terminal amidation, while overall 12 different forms of ghrelin peptides, including short form of 18-amino acid peptide and seven kinds of acyl modifications were identified. The expression profiles of the gastric ghrelin and pepsinogen 2 genes showed no significant changes related to the early growth stages. The present results suggest that digestive physiology has already been functional in this growth stage of the juvenile bluefin tuna and ghrelin may have a role in the sustained digestive and metabolic activities.

References

Apr 21, 2001·Journal of Endocrinological Investigation·G MuccioliR Deghenghi
May 29, 2001·European Journal of Endocrinology·O GualilloC Diéguez
May 16, 2002·The Journal of Endocrinology·T HayashidaN Murakami
May 7, 2003·Biochemical and Biophysical Research Communications·Ishwar S ParharYasuo Sakuma
Jul 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hiroyuki KaiyaKenji Kangawa
Mar 6, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Suraj Unniappan, Richard E Peter
Oct 16, 2004·The Journal of Clinical Endocrinology and Metabolism·Hiroyuki OsawaKentaro Sugano
Oct 23, 2004·The Journal of Experimental Biology·Jeffrey B Graham, Kathryn A Dickson
Dec 18, 2004·Endocrinology·Wei WeiGeorge H Greeley
Mar 25, 2005·Physiological Reviews·Masayasu Kojima, Kenji Kangawa
Aug 23, 2005·General and Comparative Endocrinology·Hiroyuki KaiyaKenji Kangawa
May 2, 2006·The Journal of Endocrinology·Chung-Man YeungChristopher H K Cheng
May 12, 2006·Peptides·Kouhei MatsudaSeiji Shioda
Jan 24, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brian S ShepherdGregory M Weber
Mar 17, 2007·General and Comparative Endocrinology·Akatsuki KawakoshiKenji Kangawa
Jun 29, 2007·General and Comparative Endocrinology·Genciana TerovaMarco Saroglia
Nov 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shaozhen FengHaoran Lin
Jan 9, 2008·The Journal of Endocrinology·Anthony J ManningSusan E Douglas
Jan 29, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroyuki KaiyaSuraj Unniappan
Apr 25, 2008·General and Comparative Endocrinology·Ichiro YamamotoMinoru Tanaka
Sep 13, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masao TanjiKenji Takahashi
Jun 23, 2009·General and Comparative Endocrinology·Eirik FrøilandTadahide Kurokawa
Aug 12, 2010·International Journal of Peptides·Sara E SchwandtLarry G Riley
May 24, 2011·Peptides·Hiroyuki KaiyaKenji Kangawa
Jul 16, 2011·International Journal of Peptides·Ki Sung KangKouhei Matsuda

❮ Previous
Next ❯

Citations

Apr 8, 2015·Journal of Animal Physiology and Animal Nutrition·H Volkoff
Dec 12, 2012·General and Comparative Endocrinology·Hamed Kolangi MiandareMikko Nikinmaa
Mar 23, 2017·Fish Physiology and Biochemistry·Yi SongYa-Xiong Tao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.