Identification and genotyping of Enterocytozoon bieneusi in wild Himalayan marmots (Marmota himalayana) and Alashan ground squirrels (Spermophilus alashanicus) in the Qinghai-Tibetan Plateau area (QTPA) of Gansu Province, China.

Parasites & Vectors
Jie XuYujuan Shen

Abstract

Enterocytozoon bieneusi is the most frequently detected microsporidian species in humans and animals. Currently, to the best of our knowledge, no information on E. bieneusi infection in Himalayan marmots (Marmota himalayana) and Alashan ground squirrels (Spermophilus alashanicus) is available worldwide. The aim of the present study was to understand the occurrence and genetic characterizations of E. bieneusi in Himalayan marmots and Alashan ground squirrels in the Qinghai-Tibetan Plateau area (QTPA), Gansu Province, China. A total of 498 intestinal contents were collected from 399 Himalayan marmots and 99 Alashan ground squirrels in QTPA. These samples were screened for the presence of E. bieneusi by using nested polymerase chain reaction and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. The ITS-positive sequences were aligned and phylogenetically analyzed to determine the genotypes of E. bieneusi. The average infection rate of E. bieneusi was 10.0% (50/498), with 11.8% (47/399) in Himalayan marmots and 3.0% (3/99) in Alashan ground squirrels. A total of 7 distinct E. bieneusi genotypes were confirmed: 1 known genotype, YAK1 (n = 18) and 6 novel genotypes, named as ZY37 (n = 27), H...Continue Reading

References

Apr 27, 2002·Applied and Environmental Microbiology·Michael A BuckholtSaul Tzipori
Apr 24, 2007·Applied and Environmental Microbiology·Szymon JedrzejewskiAnna C Majewska
Jun 1, 2007·Journal of Clinical Microbiology·Vitaliano A CamaLihua Xiao
Apr 2, 2009·The Journal of Eukaryotic Microbiology·Mónica Santín, Ronald Fayer
Aug 12, 2010·Research in Veterinary Science·Mónica Santín, Ronald Fayer
Mar 11, 2011·Journal of Clinical Microbiology·Xu ZhangQijun Chen
Jul 8, 2011·Epidemiology and Infection·V DecraeneM Löfdahl
Mar 24, 2012·Journal of Clinical Microbiology·Christelle PomaresFlorent Morio
Oct 22, 2013·Applied and Environmental Microbiology·Yaqiong GuoLihua Xiao
Jan 15, 2015·Parasitology International·V R S FiuzaM Santín
Jun 23, 2015·Annals of Agricultural and Environmental Medicine : AAEM·Oľga DanišováAntónia Hasajová
Nov 2, 2015·Veterinary Parasitology·Agnieszka Perec-MatysiakKinga Leśniańska
Jan 23, 2016·Trends in Parasitology·G D StentifordL Solter
Nov 25, 2016·Parasite : Journal De La Société Française De Parasitologie·Ziyin YangJianping Cao
Apr 20, 2018·PLoS Neglected Tropical Diseases·Mohammad Hasan RabieeEhsan Mostafavi
Apr 29, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Haiyan WangLongxian Zhang
May 12, 2019·Trends in Parasitology·Wei LiMonica Santin
Jul 19, 2019·International Journal for Parasitology. Parasites and Wildlife·Xiaolong HuangGuangneng Peng
Sep 9, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wei LiLihua Xiao
Sep 25, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Qingxun ZhangZiyuan Duan
Jan 17, 2020·The Journal of Eukaryotic Microbiology·Ehsan JavanmardMohammad Reza Zali

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Datasets Mentioned

BETA
MH260592
KY706128

Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

MEGA

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