Identification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans.

Journal of Bacteriology
Diliana Dancheva SimeonovaBernhard Schink

Abstract

Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphitoxidans, constructed using the fosmid vector pJK050, was screened for clones harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino acid sequences of phosphite-induced proteins. Sequence analysis of two positive clones revealed a putative operon of seven genes predicted to be involved in phosphite oxidation. Four of these genes (ptxD-ptdFCG) were cloned and heterologously expressed in Desulfotignum balticum, a related strain that cannot use phosphite as either an electron donor or as a phosphorus source. The ptxD-ptdFCG gene cluster was sufficient to confer phosphite uptake and oxidation ability to the D. balticum host strain but did not allow use of phosphite as an electron donor for chemolithotrophic growth. Phosphite oxidation activity was measured in cell extracts of D. balticum transconjugants, suggesting that all genes required for phosphite oxidation were cloned. Genes of the phosphite gene cluster were assigned putative functions on the basis of sequence anal...Continue Reading

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Citations

May 25, 2013·Genome Announcements·Anja PoehleinDiliana D Simeonova
Nov 5, 2013·BMC Genomics·Anja PoehleinDiliana D Simeonova
Sep 13, 2014·Genome Announcements·Anja PoehleinDiliana D Simeonova
Feb 13, 2016·Standards in Genomic Sciences·Anja PoehleinDiliana D Simeonova
Dec 27, 2011·Journal of Bioscience and Bioengineering·Ryuichi HirotaAkio Kuroda
Oct 19, 2011·Environmental Microbiology·Asunción MartínezSallie W Chisholm
Aug 3, 2014·Water Research·Tian-wei HaoGuang-Hao Chen
Nov 25, 2017·Nature Communications·Claudine BissonAndrew Hitchcock
Dec 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Israel A FigueroaJohn D Coates
Mar 10, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Barry P RosenTimothy R McDermott
Nov 14, 2015·Standards in Genomic Sciences·Anja PoehleinDiliana D Simeonova
Mar 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sophia D EwensJohn D Coates
Oct 25, 2017·Synthetic and Systems Biotechnology·Hironori TaniguchiKohsuke Honda

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